Molecular cloning and characterization of human pyruvate dehydrogenase. beta. subunit gene

Journal Article · Wed Aug 01 04:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.15.5594· OSTI ID:6214288

Koike, Kichiko; Urata, Yoshishige; Koike, Masahiko ^[1]

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1. Nagasaki Univ. School of Medicine (Japan)

A genomic clone encompassing the entire gene for the human pyruvate dehydrogenase {beta} subunit (PDH{beta}) has been isolated by screening a leukocyte genomic library with a nick-translated human foreskin fibroblast PDH{beta} cDNA probe. The 18-kilobase clone was characterized by restriction enzyme analysis, extensive DNA sequencing, and primer-extension analysis. The PDH{beta} structural gene is composed of 10 exons and 9 introns. All intron-exon splice junctions follow the GT/AG rule. The Alu family was found in introns 2 and 8. The 5{prime} flanking region of the PDH{beta} gene contains a CAAT consensus promoter sequence but no TATA sequence. Primer-extension analysis indicated the PDH{beta} gene transcription start site is an adenine residue located 132 bases upstream from the initiation codon in exon 1.

• OSTI ID: 6214288
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 87:15; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ADENINES
AMINES
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
AROMATICS
AZAARENES
AZINES
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CLONING
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DNA
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
DRUGS
ENZYMES
FIBROBLASTS
GENES
HEMIACETAL DEHYDROGENASES
HETEROCYCLIC COMPOUNDS
HYBRIDIZATION
HYDROXY COMPOUNDS
ISOTOPES
KETO ACIDS
LIGHT NUCLEI
MAMMALS
MAN
MESSENGER-RNA
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
OXIDOREDUCTASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
PURINES
PYRIMIDINES
PYRUVIC ACID
RADIOISOTOPES
RECOMBINANT DNA
RNA
SKIN
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
THIAMINE
THIAZOLES
TRANSCRIPTION
VERTEBRATES
VITAMIN B GROUP
VITAMINS