Effect of sodium intake on gene expression and plasma levels of ANF in rats

Journal Article · Mon Aug 01 00:00:00 EDT 1988 · American Journal of Physiology; (USA)

OSTI ID:6940670

Lattion, A L; Aubert, J F; Flueckiger, J P; Nussberger, J; Waeber, B; Brunner, H R ^[1]

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1. Centre Hospitalier Universitaire Vaudois, Lausanne (Switzerland)

The effect of short- and long-term sodium loading and sodium restriction on the gene expression as well as on circulating plasma levels of atrial natriuretic factor (ANF) was evaluated in normotensive Wistar rats. These rats were fed either a low-, a regular-, or a high-sodium diet (regular diet and 1% saline as drinking fluid) and studied after 1 and 3 wk. The ANF mRNA was determined in pooled atria and ventricles of the different groups of rats, using the dot-blot technique. Plasma ANF levels were measured with a radioimmunoassay. After 1 wk on the high-sodium diet, ANF mRNA was increased in right atria and ventricles together with circulating ANF levels when compared with animals maintained for the same period on a low-sodium diet. After 3 wk on the various diets, the differences in cardiac ANF mRNA and in plasma ANF levels had disappeared. Gene expression of ANF was also looked for in different areas of the brain, lung, thyroid, adrenals, and the kidney; no hybridization was detected in any of these organs. These data suggest that in rats, the transcription of the ANF gene and peptide release is enhanced only during short-term adaptation to dietary sodium loading.

• OSTI ID: 6940670
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 255:2; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

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550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOASSAY
BIOLOGICAL EFFECTS
BODY
CARDIOVASCULAR SYSTEM
CATIONS
CHARGED PARTICLES
CHLORIDES
CHLORINE COMPOUNDS
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DIET
DIGESTIVE SYSTEM
DISTRIBUTION
DNA HYBRIDIZATION
ELECTRON CAPTURE RADIOISOTOPES
GENE REGULATION
GLANDS
HALIDES
HALOGEN COMPOUNDS
HEART
HORMONES
HYBRIDIZATION
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
IONS
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LIGHT NUCLEI
LIVER
MALES
MAMMALS
MESSENGER-RNA
MUSCLES
MYOCARDIUM
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RADIOISOTOPES
RATS
RNA
RODENTS
SALIVARY GLANDS
SODIUM CHLORIDES
SODIUM COMPOUNDS
TISSUE DISTRIBUTION
TRACER TECHNIQUES
TRANSCRIPTION
VERTEBRATES