Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Atrial natriuretic factor: radioimmunoassay and effects on adrenal and pituitary glands

Journal Article · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5509459
; ; ; ; ; ; ; ; ;

A simple and sensitive radioimmunoassay was developed for measurement of immunoreactive atrial natriuretic factor (IR-ANF) in rat and human plasma and in rat atria. The two atria contain about 20 ..mu..g ANF per rat. The right atrium contained 2.5 times more ANF than did the left. Ether anesthesia and morphine markedly increased IR-ANF in rat plasma. The concentration of IR-ANF in plasma of clinically normal human subjects was 65.3 +/- 2.5 pg/ml. Paroxysmal tachycardia and rapid atrial pacing significantly increased IR-ANF in human plasma. Two- to seven-fold higher concentrations were found in coronary sinus blood than in the peripheral circulation. In the plasma of rats and humans, circulating ANF is probably a small-molecular-weight peptide. ANF acts on the adrenal and the pituitary. ANF inhibits aldosterone secretion from rat zona glomerulosa and steroid secretion by bovine adrenal zona glomerulosa and fasciculata. ANF stimulated the basal secretion of arginine vasopressin (AVP) in vitro and inhibited KCl-stimulated release of AVP.

Research Organization:
Clinical Research Institute of Montreal, Quebec
OSTI ID:
5509459
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:7; ISSN FEPRA
Country of Publication:
United States
Language:
English

Similar Records

Atrial natriuretic factor-like activity in rat posterior pituitary
Conference · Tue Mar 04 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) · OSTI ID:6763798
Alpha-human atrial natriuretic polypeptide (. cap alpha. -hANP) specific binding sites in bovine adrenal gland
Journal Article · Fri Jun 13 00:00:00 EDT 1986 · Biochem. Biophys. Res. Commun.; (United States) · OSTI ID:7108792
Atrial natriuretic factor binding sites in experimental congestive heart failure
Journal Article · Sun Oct 01 00:00:00 EDT 1989 · American Journal of Physiology; (USA) · OSTI ID:5259840

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL GLANDS
ADRENAL HORMONES
ALDEHYDES
ALDOSTERONE
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY
BODY FLUIDS
CORTICOSTEROIDS
ENDOCRINE GLANDS
GLANDS
HORMONES
HYDROXY COMPOUNDS
IMMUNE REACTIONS
IMMUNOASSAY
IMMUNOLOGY
INHIBITION
ISOTOPE APPLICATIONS
KETONES
MAMMALS
MAN
MATERIALS
MINERALOCORTICOIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PEPTIDES
PITUITARY GLAND
PITUITARY HORMONES
PREGNANES
PRIMATES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RATS
RODENTS
SECRETION
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
VASOPRESSIN
VERTEBRATES