Region-specific microdissection library and single-copy microclones for human chromosome 2p11-p13
- Univ. of Colorado Health Sciences Center, Denver, CO (United States)
The authors report the construction and characterization of a region-specific microdissection library for human chromosome 2p11-p13. This library (designated 2P4 library) is large, comprising 600,000 recombinant microclones. Thirty to 40% of the clones contain unique sequences. The insert sizes range from 100 to 800 bp, with a mean of 380 bp. A subset of the microclones was selected, based on their weak or no hybridization to total human DNA, for further analysis. Of 50 single-copy microclones analyzed, 35 clones (70%) were derived from human and are chromosome 2-specific. The insert sizes and the hybridizing genomic HindIII fragments of these clones were also determined. The 2P3 microdissection library and the single-copy microclones from the library are useful in preparing STS (sequence-tagged site) to isolate corresponding YAC (yeast artificial chromosome) or other clones with large inserts and for isolating region-specific cDNA clones as candidate genes for cloning disease-related genes assigned to this region.
- DOE Contract Number:
- FG03-94ER61819
- OSTI ID:
- 6940635
- Journal Information:
- Somatic Cell and Molecular Genetics; (United States), Vol. 20:2, Issue 2; ISSN 0740-7750
- Country of Publication:
- United States
- Language:
- English
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