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Title: Region-specific microdissection library and single-copy microclones for human chromosome 2p11-p13

Abstract

The authors report the construction and characterization of a region-specific microdissection library for human chromosome 2p11-p13. This library (designated 2P4 library) is large, comprising 600,000 recombinant microclones. Thirty to 40% of the clones contain unique sequences. The insert sizes range from 100 to 800 bp, with a mean of 380 bp. A subset of the microclones was selected, based on their weak or no hybridization to total human DNA, for further analysis. Of 50 single-copy microclones analyzed, 35 clones (70%) were derived from human and are chromosome 2-specific. The insert sizes and the hybridizing genomic HindIII fragments of these clones were also determined. The 2P3 microdissection library and the single-copy microclones from the library are useful in preparing STS (sequence-tagged site) to isolate corresponding YAC (yeast artificial chromosome) or other clones with large inserts and for isolating region-specific cDNA clones as candidate genes for cloning disease-related genes assigned to this region.

Authors:
; ;  [1]
  1. (Univ. of Colorado Health Sciences Center, Denver, CO (United States))
Publication Date:
OSTI Identifier:
6940635
DOE Contract Number:  
FG03-94ER61819
Resource Type:
Journal Article
Journal Name:
Somatic Cell and Molecular Genetics; (United States)
Additional Journal Information:
Journal Volume: 20:2; Journal Issue: 2; Journal ID: ISSN 0740-7750
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; HUMAN CHROMOSOME 2; GENETIC MAPPING; DNA HYBRIDIZATION; DNA SEQUENCING; DNA-CLONING; BIOTECHNOLOGY; CHROMOSOMES; CLONING; GENETIC ENGINEERING; HUMAN CHROMOSOMES; HYBRIDIZATION; MAPPING; NUCLEIC ACID HYBRIDIZATION; STRUCTURAL CHEMICAL ANALYSIS; 550400* - Genetics

Citation Formats

Yu, Jingwei, Qi, Jianxin, and Kao, Fa-Ten. Region-specific microdissection library and single-copy microclones for human chromosome 2p11-p13. United States: N. p., 1994. Web. doi:10.1007/BF02290682.
Yu, Jingwei, Qi, Jianxin, & Kao, Fa-Ten. Region-specific microdissection library and single-copy microclones for human chromosome 2p11-p13. United States. doi:10.1007/BF02290682.
Yu, Jingwei, Qi, Jianxin, and Kao, Fa-Ten. Tue . "Region-specific microdissection library and single-copy microclones for human chromosome 2p11-p13". United States. doi:10.1007/BF02290682.
@article{osti_6940635,
title = {Region-specific microdissection library and single-copy microclones for human chromosome 2p11-p13},
author = {Yu, Jingwei and Qi, Jianxin and Kao, Fa-Ten},
abstractNote = {The authors report the construction and characterization of a region-specific microdissection library for human chromosome 2p11-p13. This library (designated 2P4 library) is large, comprising 600,000 recombinant microclones. Thirty to 40% of the clones contain unique sequences. The insert sizes range from 100 to 800 bp, with a mean of 380 bp. A subset of the microclones was selected, based on their weak or no hybridization to total human DNA, for further analysis. Of 50 single-copy microclones analyzed, 35 clones (70%) were derived from human and are chromosome 2-specific. The insert sizes and the hybridizing genomic HindIII fragments of these clones were also determined. The 2P3 microdissection library and the single-copy microclones from the library are useful in preparing STS (sequence-tagged site) to isolate corresponding YAC (yeast artificial chromosome) or other clones with large inserts and for isolating region-specific cDNA clones as candidate genes for cloning disease-related genes assigned to this region.},
doi = {10.1007/BF02290682},
journal = {Somatic Cell and Molecular Genetics; (United States)},
issn = {0740-7750},
number = 2,
volume = 20:2,
place = {United States},
year = {1994},
month = {3}
}