Uptake and metabolism of sugars by suspension-cultured catharanthus roseus cells

Journal Article · Sat Apr 01 04:00:00 EST 1989 · Plant Physiology, Supplement; (USA)

OSTI ID:6939281

Ashihara, Hiroshi; Sagishima, Kyoko; Kubota, Kaoru ^[1]

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1. Ochanomizu Univ., Tokyo (Japan)

The Uptake and metabolism of sugars by suspension-cultured Catharanthus roseus cells were investigated. Substantially all the sucrose in the culture medium was hydrolyzed to glucose and fructose before being taken up by the cells. The activity of invertase bound to cell walls, determined in situ, was high at the early stage of culture. Glucose was more easily taken up by the cells than was fructose. Tracer experiments using (U-{sup 14}C)glucose and (U-{sup 14}C)fructose indicated that glucose is a better precursor for respiration than fructose, while fructose is preferentially utilized for the synthesis of sucrose, especially in the early phase of cell growth. These results suggest that fructose is utilized for the synthesis of sucrose via the reaction catalyzed by sucrose synthase, prior to the phosphorylation by hexokinase or fructokinase.

• OSTI ID: 6939281
• Journal Information: Plant Physiology, Supplement; (USA), Journal Name: Plant Physiology, Supplement; (USA) Vol. 89:4; ISSN PPYSA; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
CARBOHYDRATES
CARBON 14 COMPOUNDS
CHEMICAL REACTIONS
DISACCHARIDES
FRUCTOSE
GLUCOSE
HEXOSES
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
METABOLISM
MONOSACCHARIDES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PHOSPHORYLATION
PLANT CELLS
RESPIRATION
SACCHARIDES
SACCHAROSE
TRACER TECHNIQUES
UPTAKE