Evidence for the presence of a sucrose carrier in immature sugar-beet roots
Conference
·
· Plant Physiol., Suppl.; (United States)
OSTI ID:5625251
Unlike in mature sugar-beet roots, sucrose is assumed to be hydrolyzed by a wall-bound invertase prior to uptake by immature roots. To test this hypothesis, they used a sucrose analog, 1'fluorosucrose which is recognized by the carrier but is a poor substrate for invertases. Asymmetrically labeled sucrose (/sup 3/H-fructose) 1'fluorosucrose (/sup 14/C-glucose) were applied at 10 mM (/sup 3/H//sup 14/C=1) to an attached source leaf. After 6 h, sugars from plant parts in the translocation path were separated on HPLC. /sup 14/C-1'fluorosucrose was translocated and accumulated in the root at a higher rate than /sup 3/H-sucrose due to greater metabolism of /sup 3/H-sucrose in the shoot (indicated by the presence of /sup 3/H in hexose fractions and loss of asymmetry). In the root 25% of the /sup 3/H-sucrose was hydrolyzed to hexoses whereas no /sup 14/C was detected in hexose fractions. The data indicate that despite high cell-wall invertase and cytoplasmic sucrose synthase activities, young sugar-beet roots import and store sucrose without hydrolysis. Therefore, the function of a group translocator at the tonoplast is unclear.
- Research Organization:
- Rutgers, The State Univ. of New Jersey, New Brunswick
- OSTI ID:
- 5625251
- Report Number(s):
- CONF-8707108-
- Conference Information:
- Journal Name: Plant Physiol., Suppl.; (United States) Journal Volume: 83:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARRIERS
CELL CONSTITUENTS
CELL WALL
CHEMICAL REACTIONS
CHROMATOGRAPHY
DECOMPOSITION
DISACCHARIDES
DISTRIBUTION
ENZYME ACTIVITY
FOOD
HYDROLYSIS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEAVES
LIQUID COLUMN CHROMATOGRAPHY
LYSIS
METABOLISM
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
ROOT ABSORPTION
ROOTS
SACCHARIDES
SACCHAROSE
SEPARATION PROCESSES
SOLVOLYSIS
TISSUE DISTRIBUTION
TRACER TECHNIQUES
TRANSLOCATION
TRITIUM COMPOUNDS
UPTAKE
VEGETABLES
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARRIERS
CELL CONSTITUENTS
CELL WALL
CHEMICAL REACTIONS
CHROMATOGRAPHY
DECOMPOSITION
DISACCHARIDES
DISTRIBUTION
ENZYME ACTIVITY
FOOD
HYDROLYSIS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEAVES
LIQUID COLUMN CHROMATOGRAPHY
LYSIS
METABOLISM
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
ROOT ABSORPTION
ROOTS
SACCHARIDES
SACCHAROSE
SEPARATION PROCESSES
SOLVOLYSIS
TISSUE DISTRIBUTION
TRACER TECHNIQUES
TRANSLOCATION
TRITIUM COMPOUNDS
UPTAKE
VEGETABLES