A preliminary report on zinc-induced resistance to nitrogen mustard toxicity in mice

Technical Report · Thu Dec 01 00:00:00 EST 1988

OSTI ID:6939034

Shackelford, M E; Tobey, R A

Previous studies with cultured human normal fibroblasts indicated that treatment of cells with zinc before exposure to alkylating agents enhanced cell survival by seven- to nine-fold. To establish whether a similar zinc-induced protective response could be elicited in vivo, a set of preliminary experiments was carried out in which Balb/cJ mice were treated with zinc chloride (2 mg/kg body weight) or saline by intraperitoneal (ip) injection at 48, 36, 24, and 12 h before ip administration of 4 mg/kg of the alkylating agent nitrogen mustard. Of the animals that received saline before nitrogen mustard, 57% were killed compared with only 20% in the group treated with zinc before administration of the alkylating agent. These results (which almost certainly were achieved with less than optimal induction conditions) provide evidence for the existence, in vivo, of a zinc-inducible process that reduces alkylating agent lethality. 12 refs., 4 figs.

• Research Organization: Los Alamos National Lab., NM (USA)
• DOE Contract Number: W-7405-ENG-36
• OSTI ID: 6939034
• Report Number(s): LA-11447; ON: DE89003458
• Resource Relation: Other Information: Portions of this document are illegible in microfiche products
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CELL KILLING
MINIMIZATION
DOSE-RESPONSE RELATIONSHIPS
MODIFICATIONS
NITROGEN MUSTARD
TOXICITY
ZINC CHLORIDES
BIOCHEMICAL REACTION KINETICS
ALKYLATING AGENTS
DISEASE RESISTANCE
FIBROBLASTS
LETHAL DOSES
MICE
RESPONSE MODIFYING FACTORS
AMINES
ANIMAL CELLS
ANIMALS
CHLORIDES
CHLORINE COMPOUNDS
CONNECTIVE TISSUE CELLS
DOSES
HALIDES
HALOGEN COMPOUNDS
KINETICS
MAMMALS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
REACTION KINETICS
RODENTS
SOMATIC CELLS
VERTEBRATES
ZINC COMPOUNDS
ZINC HALIDES
560300* - Chemicals Metabolism & Toxicology