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Clastogenic effects of transplacental exposure of mouse embryos to nitrogen mustard of cyclophosphamide

Journal Article · · Teratog., Carcinog., Mutagen.; (United States)
Embryos from day 12 pregnant Swiss mice given intraperitoneal injections of nitrogen mustard (HN/sub 2/) or cyclophosphamide (CP) were evaluated for chromosomal aberrations. Both agents induced dose-dependent increases in the frequency of cells with aberrations observed in embryos from females treated 6 hr before sacrifice. The highest frequencies of cells with aberrations were observed when females were injected 15 or 18 hr before sacrifice on day 12. A teratogenic dose of HN/sub 2/ (1.0 mg/kg) induced significantly higher frequencies of damaged cells than a teratogenic dose of CP (20 mg/kg). Cytogenetic analysis of rodent embryos from pregnant females exposed to xenobiotic agents may be an effective screening test for evaluation of genetic effects induced by transplacental exposure.
Research Organization:
Los Alamos National Lab., NM
OSTI ID:
6979808
Journal Information:
Teratog., Carcinog., Mutagen.; (United States), Journal Name: Teratog., Carcinog., Mutagen.; (United States) Vol. 3; ISSN TCMUD
Country of Publication:
United States
Language:
English