Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Galanin inhibits acetylcholine release in the ventral hippocampus of the rat: histochemical, autoradiographic, in vivo, and in vitro studies

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
; ; ; ; ; ; ;

A high density of galanin binding sites was found by using /sup 125/I-labeled galanin, iodinated by chloramine-T, followed by autoradiography in the ventral, but not in the dorsal, hippocampus of the rat. Lesions of the fimbria and of the septum caused disappearance of a major population of these binding sites, suggesting that a large proportion of them is localized on cholinergic nerve terminals of septal afferents. As a functional correlate to these putative galanin receptor sites, it was shown, both in vivo and in vitro, that galanin, in a concentration-dependent manner, inhibited the evoked release of acetylcholine in the ventral, but not in the dorsal, hippocampus. Intracerebroventricularly applied galanin fully inhibited the scopolamine stimulated release of acetylcholine in the ventral, but not in the dorsal, hippocampus, as measured by the microdialysis technique. In vitro, galanin inhibited the 25 mM K/sup +/-evoked release of (/sup 3/H)acetylcholine from slices of the ventral hippocampus, with an IC/sub 50/ value of approx. = 50 nM. These results are discussed with respect to the colocalization of galanin- and choline acetyltransferase-like immunoreactivity in septal somata projecting to the hippocampus.

Research Organization:
Istituto di Ricerche Farmacologiche Mario Negri, Milano (Italy)
OSTI ID:
6938571
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:20; ISSN PNASA
Country of Publication:
United States
Language:
English

Similar Records

N-terminal galanin-(1-16) fragment is an agonist at the hippocampal galanin receptor
Journal Article · Thu Nov 30 23:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA) · OSTI ID:5031339
Changes in acetylcholine content, release and muscarinic receptors in rat hippocampus under cold stress
Journal Article · Sat Dec 31 23:00:00 EST 1988 · Life Sci.; (United States) · OSTI ID:5857413
Autoradiographic distribution of /sup 125/I-galanin binding sites in the rat central nervous system
Journal Article · Fri Oct 31 23:00:00 EST 1986 · Peptides (Fayetteville, N.Y.); (United States) · OSTI ID:6619632

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ACETYLCHOLINE
ALKALI METAL COMPOUNDS
AMINES
AMMONIUM COMPOUNDS
AUTONOMIC NERVOUS SYSTEM AGENTS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOCHEMISTRY
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CHEMISTRY
CYTOCHEMISTRY
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ESTERS
HALIDES
HALOGEN COMPOUNDS
HIPPOCAMPUS
INORGANIC PHOSPHORS
INTERMEDIATE MASS NUCLEI
IODIDES
IODINE 125
IODINE COMPOUNDS
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
MEMBRANE PROTEINS
NERVOUS SYSTEM
NEUROREGULATORS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PARASYMPATHOMIMETICS
PEPTIDES
PHOSPHORS
PROTEINS
QUATERNARY COMPOUNDS
RADIOISOTOPES
RECEPTORS
SODIUM COMPOUNDS
SODIUM IODIDES
TRITIUM COMPOUNDS