Changes in acetylcholine content, release and muscarinic receptors in rat hippocampus under cold stress
The aim was to study the mechanism of the previously established decrease in acetylcholine (ACh) concentration in the rat hippocampus under cold stress. Male rats were exposed for 14 days to cold (5/degree/C) or kept (controls) at room temperature (24/degree/C). Acetylcholine content, release and muscarinic receptor binding were investigated in the hippocampus. Cold exposure resulted in a decrease of ACh concentration in the dorsal hippocampus. Moreover, the potassium-evoked release of ACh from hippocampal slices was increased and an increase of maximal binding capacity of (/sup 3/H)(-) quinuclidinyl benzilate in the dorsal hippocampus of cold exposed animals was also observed. Thus the decrease of hippocampal ACh concentration under cold exposure is probably due to its increased release. On balance then, our results demonstrate that cold stress in the rat induces significant activation of the hippocampal cholinergic system.
- Research Organization:
- Institute of Experimental Endocrinology, Bratislava (Czechoslovakia)
- OSTI ID:
- 5857413
- Journal Information:
- Life Sci.; (United States), Vol. 45:2
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
BIOSYNTHESIS
HIPPOCAMPUS
TEMPERATURE EFFECTS
BIOCHEMICAL REACTION KINETICS
RATS
RECEPTORS
SECRETION
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
DRUGS
ESTERS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANS
PARASYMPATHOMIMETICS
PROTEINS
QUATERNARY COMPOUNDS
REACTION KINETICS
RODENTS
SYNTHESIS
VERTEBRATES
560200* - Thermal Effects
550201 - Biochemistry- Tracer Techniques