Insulin response in individual tissues of control and gold thioglucose-obese mice in vivo with (1-/sup 14/C)2-deoxyglucose
The dose-response characteristics of several glucose-utilizing tissues (brain, heart, white adipose tissue, brown adipose tissue, and quadriceps muscle) to a single injection of insulin have been compared in control mice and mice made obese with a single injection of gold thioglucose (GTG). Tissue content of (1-/sup 14/C)2-deoxyglucose 6-phosphate and blood disappearance rate of (1-/sup 14/C)2-deoxyglucose (2-DG) were measured at nine different insulin doses and used to calculate rates of 2-DG uptake and phosphorylation in tissues from control and obese mice. The insulin sensitivity of tissues reflected in the ED50 of insulin response varied widely, and brown adipose tissue was the most insulin-sensitive tissue studied. In GTG-obese mice, heart, quadriceps, and brown adipose tissue were insulin resistant (demonstrated by increased ED50), whereas in white adipose tissue, 2-DG phosphorylation was more sensitive to insulin. Brain 2-DG phosphorylation was insulin independent in control and obese animals. The largest decrease in insulin sensitivity in GTG-obese mice was observed in brown adipose tissue. The loss of diet-induced thermogenesis in brown adipose tissue as a result of the hypothalamic lesion in GTG-obese mice could be a major cause of insulin resistance in brown adipose tissue. Because brown adipose tissue can make a major contribution to whole-body glucose utilization, insulin resistance in this tissue may have a significant effect on whole-animal glucose homeostasis in GTG-obese mice.
- Research Organization:
- Royal Prince Alfred Hospital, Camperdown, New South Wales, Australia
- OSTI ID:
- 6937409
- Journal Information:
- Diabetes; (United States), Vol. 2
- Country of Publication:
- United States
- Language:
- English
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INSULIN
TISSUE DISTRIBUTION
ADIPOSE TISSUE
BRAIN
CARBON 14 COMPOUNDS
GLUCOSE
GOLD
HEART
METABOLIC DISEASES
MICE
MUSCLES
MYOCARDIUM
PHOSPHORYLATION
TRACER TECHNIQUES
ALDEHYDES
ANIMAL TISSUES
ANIMALS
BODY
CARBOHYDRATES
CARDIOVASCULAR SYSTEM
CENTRAL NERVOUS SYSTEM
CHEMICAL REACTIONS
CONNECTIVE TISSUE
DISEASES
DISTRIBUTION
ELEMENTS
HEXOSES
HORMONES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
METALS
MONOSACCHARIDES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
RODENTS
SACCHARIDES
TISSUES
TRANSITION ELEMENTS
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques