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Evidence for dual cyclooxygenases in mouse and rat brain

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6937045
; ;
The existence of dual forms of cyclooxygenase (CO) in the whole brain of rat and mouse was investigated. Using microsomes prepared from tissue homogenized in 10 mM EDTA and 1% BSA, they assayed for prostaglandin (PG) in a medium containing 1-(/sup 14/C)-arachidonic acid (AA: 1 ..mu..g; 300,000 cpm) 1.2 mM epinephrine, and 1 mM glutathione. The mouse microsomal PGE/sub 2/ synthesis rose rapidly and plateaued within 5 minutes while PGF/sub 2..cap alpha../ levels continued to rise through the 60 minute incubation. Evidence for the existence of two forms of the CO in the mouse brain came from the observations that (1) 0.4 ..mu..M indomethacin inhibited PGE/sub 2/ production) by 80% while PGF/sub 2..cap alpha../ synthesis decreased only 20% and (2) a 3 minute preincubation of the mouse microsomes with unlabelled AA (1 ..mu.. g) eliminated PGE/sub 2/ synthesis while PGF/sub 2..cap alpha../ synthesis continued. Similar results were obtained with rat brain microsomes. Rat kidney microsomal preparations appear not to have the two CO forms. From these observations, it is concluded that there are PGE/sub 2/ and PGF/sub 2..cap alpha../ associated CO in mouse and rat brain microsomal preparations. The PGF/sub 2..cap alpha../ associated CO is somewhat resistant to arachidonate induced destruction while the PGE/sub 2/ associated CO undergoes autodestruction readily.
Research Organization:
New Jersey Medical School, Newark
OSTI ID:
6937045
Report Number(s):
CONF-8606151-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ADRENALINE
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOASSAY
BIOSYNTHESIS
BODY
BRAIN
CARBON 14 COMPOUNDS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
DRUGS
ENZYMES
GLUTATHIONE
HORMONES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MICE
MICROSOMES
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
OXIDOREDUCTASES
OXYGENASES
PEPTIDES
POLYPEPTIDES
PROSTAGLANDINS
PROTEINS
RADIOPROTECTIVE SUBSTANCES
RATS
RODENTS
STEROID HORMONES
SYMPATHOMIMETICS
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES