Production of additional metabolites of arachidonic acid by rapidly growing hepatomas

Conference · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5020931

Cyran, J; Lea, M A; Lysz, T W

There is evidence that tumor growth may be regulated by prostaglandins (PGs). They examined the metabolism of (1-/sup 14/C)arachidonic acid by rat hepatomas of the Morris series in order to determine if PG synthesis is correlated with tumor growth rate. The PG synthesis by microsomal fractions of rapidly growing hepatomas (7288CTC, 7777 and 7800) was more extensive than with preparations from hepatomas of intermediate (5123C and 8999) or slow growth rate (20 and 7787). The principal products were PGF/sub 2..cap alpha../, PGE/sub 2/, thromboxane B/sub 2/ (TXB/sub 2/) and hydroxy fatty acids (OH-FA). Features characteristic of the rapidly growing hepatomas were the production of TXB/sub 2/ and a OH-FA whose synthesis was inhibited by 1 ..mu..M indomethacin. PGD/sub 2/ was found only in the microsomal preparations from large tumors and their associated host livers. Formation of these icosanoids was not detected with microsomes from control rat liver or regenerating liver (24 hrs after partial hepatectomy). Production of TXB/sub 2/ was observed with microsomes from the livers of rats bearing rapidly growing tumors. Synthesis of TXB/sub 2/ was inhibited by 3 ..mu..M OKY-1581 (sodium-3-(4-3-pyridylmethyl)phenyl-3-methyl-acrylate) and the action of this compound may serve to probe the significance of enhanced synthesis of TXB/sub 2/ in rapidly growing hepatomas.

• Research Organization: New Jersey Medical School, Newark
• OSTI ID: 5020931
• Report Number(s): CONF-8606151-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ARACHIDONIC ACID
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
DISEASES
GROWTH
HEPATOMAS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
METABOLISM
METABOLITES
MICROSOMES
MONOCARBOXYLIC ACIDS
NEOPLASMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
PROSTAGLANDINS
RATS
RODENTS
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES