Induction of dexamethasone (DM) of histidine decarboxylase (HDC) in mast cells

Conference · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6936723

Ichikawa, A; Imanishi, N; Nakayama, T; Asano, M; Tomita, K

Effects of glucocorticoids on HDC in cultured mouse mastocytoma P-815 cells and rat peritoneal mast cells (RPMC) were investigated to explore the role of steroids in inflammatory tissues. DM (1 nM to 10 ..mu..M) significantly elevated the histamine content and HDC activity of P-815 cells (37/sup 0/C, 24 hrs), accompanying with a growth retardation of the cells by about 40%. In contrast to histamine, serotonin levels of P-815 cells were decreased by treatment with DM. However, DM had no significant effects on the activities of various enzymes other than HDC present in granules or membrane of P-815 cells. DM-induced increases of histamine and HDC activity were completely suppressed by the addition of cycloheximide and actinomycin D. P-815 cells were found to have the binding sites for /sup 3/H-DM in the cytosol (Kd=2.2 nM, 450 sites/cell) and in the nuclei (Kd=0.1 nM, 39 sites/nucleus). Purified HDC from P-815 cells was identified to be an isozyme of mast cell type enzyme (MW=110K, pI=5.4). In contrast, the basal histamine level of cultured RPMC was not affected by treatment of DM, which suppressed histamine release activity induced by DNP-ascaris antiserum by 40%-50%. Histamine-depleted RPMC after degranulation partially recovered histamine level by 50%-60% in the presence of DM. These results showed that glucocorticoids specifically stimulated histamine formation with the increased de novo synthesis of HDC in mast cells.

• Research Organization: Kyoto Univ., Japan
• OSTI ID: 6936723
• Report Number(s): CONF-8606151-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
AMINES
ANIMAL CELLS
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
AZOLES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CARBON-CARBON LYASES
CARBOXY-LYASES
CONNECTIVE TISSUE CELLS
CORTICOSTEROIDS
DECARBOXYLASES
DEXAMETHASONE
DRUGS
ENZYME ACTIVITY
ENZYMES
GLUCOCORTICOIDS
HETEROCYCLIC COMPOUNDS
HISTAMINE
HORMONES
HYDROXY COMPOUNDS
IMIDAZOLES
INDOLES
INFLAMMATION
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
LYASES
MAMMALS
MAST CELLS
MEMBRANE PROTEINS
MICE
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PATHOLOGICAL CHANGES
PREGNANES
PROTEINS
PYRROLES
RADIOPROTECTIVE SUBSTANCES
RATS
RECEPTORS
RODENTS
SEROTONIN
SOMATIC CELLS
STEROID HORMONES
STEROIDS
SYMPATHOMIMETICS
SYMPTOMS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TRYPTAMINES
VERTEBRATES