Induction of dexamethasone (DM) of histidine decarboxylase (HDC) in mast cells
Effects of glucocorticoids on HDC in cultured mouse mastocytoma P-815 cells and rat peritoneal mast cells (RPMC) were investigated to explore the role of steroids in inflammatory tissues. DM (1 nM to 10 ..mu..M) significantly elevated the histamine content and HDC activity of P-815 cells (37/sup 0/C, 24 hrs), accompanying with a growth retardation of the cells by about 40%. In contrast to histamine, serotonin levels of P-815 cells were decreased by treatment with DM. However, DM had no significant effects on the activities of various enzymes other than HDC present in granules or membrane of P-815 cells. DM-induced increases of histamine and HDC activity were completely suppressed by the addition of cycloheximide and actinomycin D. P-815 cells were found to have the binding sites for /sup 3/H-DM in the cytosol (Kd=2.2 nM, 450 sites/cell) and in the nuclei (Kd=0.1 nM, 39 sites/nucleus). Purified HDC from P-815 cells was identified to be an isozyme of mast cell type enzyme (MW=110K, pI=5.4). In contrast, the basal histamine level of cultured RPMC was not affected by treatment of DM, which suppressed histamine release activity induced by DNP-ascaris antiserum by 40%-50%. Histamine-depleted RPMC after degranulation partially recovered histamine level by 50%-60% in the presence of DM. These results showed that glucocorticoids specifically stimulated histamine formation with the increased de novo synthesis of HDC in mast cells.
- Research Organization:
- Kyoto Univ., Japan
- OSTI ID:
- 6936723
- Report Number(s):
- CONF-8606151-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DECARBOXYLASES
ENZYME ACTIVITY
DEXAMETHASONE
BIOLOGICAL EFFECTS
RECEPTORS
GLUCOCORTICOIDS
HISTAMINE
BIOSYNTHESIS
INFLAMMATION
MAST CELLS
MICE
RATS
SEROTONIN
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ADRENAL HORMONES
AMINES
ANIMAL CELLS
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
AZOLES
CARBON-CARBON LYASES
CARBOXY-LYASES
CONNECTIVE TISSUE CELLS
CORTICOSTEROIDS
DRUGS
ENZYMES
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROXY COMPOUNDS
IMIDAZOLES
INDOLES
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
LYASES
MAMMALS
MEMBRANE PROTEINS
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PATHOLOGICAL CHANGES
PREGNANES
PROTEINS
PYRROLES
RADIOPROTECTIVE SUBSTANCES
RODENTS
SOMATIC CELLS
STEROID HORMONES
STEROIDS
SYMPATHOMIMETICS
SYMPTOMS
SYNTHESIS
TRYPTAMINES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques