Radiation inactivation probe of membrane-bound enzymes: gamma-glutamyltranspeptidase, aminopeptidase N, and sucrase
Journal Article
·
· Anal. Biochem.; (United States)
gamma-Glutamyltranspeptidase (GGT), aminopeptidase N (AP-N), and sucrase in purified rabbit intestinal brush border membrane vesicles were irradiated in situ at -135 degrees C using high energy electrons. Surviving activities of the enzymes were measured as a function of radiation dose, and the functional unit target sizes (corresponding to carbohydrate-free polypeptides) were determined using target analysis. The in situ functional unit sizes were GGT 59 kDa, AP-N 59 kDa, and sucrase 63 kDa. Together with biochemical data determined previously, it is concluded that the noncovalently attached large (approximately 40 kDa) and small (approximately 25 kDa) subunits of GGT are both required for catalytic activity. Furthermore, these data suggest that (i) the membrane-bound form of AP-N consists of one or more noncovalently attached subunits of 59 kDa, each of which is enzymatically active; and (ii) in situ sucrase activity is associated with a subunit of 63 kDa which is noncovalently attached within the sucrase-isomaltase complex.
- Research Organization:
- Univ. of Florida College of Medicine, Gainesville
- OSTI ID:
- 6936515
- Journal Information:
- Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 2; ISSN ANBCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINOPEPTIDASES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CELL CONSTITUENTS
CELL MEMBRANES
DIGESTIVE SYSTEM
DOSE-RESPONSE RELATIONSHIPS
ENZYME ACTIVITY
ENZYMES
GASTROINTESTINAL TRACT
HYDROLASES
IN VITRO
INACTIVATION
INTESTINES
MAMMALS
MEMBRANES
ORGANS
PEPTIDE HYDROLASES
RABBITS
RADIATION EFFECTS
VERTEBRATES
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINOPEPTIDASES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CELL CONSTITUENTS
CELL MEMBRANES
DIGESTIVE SYSTEM
DOSE-RESPONSE RELATIONSHIPS
ENZYME ACTIVITY
ENZYMES
GASTROINTESTINAL TRACT
HYDROLASES
IN VITRO
INACTIVATION
INTESTINES
MAMMALS
MEMBRANES
ORGANS
PEPTIDE HYDROLASES
RABBITS
RADIATION EFFECTS
VERTEBRATES