Functional size of acyl coenzyme A:diacylglycerol acyltransferase by radiation inactivation

Journal Article · Wed Nov 01 04:00:00 EST 1989 · Journal of Lipid Research; (USA)

OSTI ID:6992798

Ozasa, S; Kempner, E S; Erickson, S K ^[1]

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1. Stanford Univ. School of Medicine, CA (USA)

Rat liver acyl coenzyme A:diacylglycerol acyltransferase, an intrinsic membrane activity associated with the endoplasmic reticulum, catalyzes the terminal and rate-limiting step in triglyceride synthesis. This enzyme has never been purified nor has its gene been isolated. Inactivation by ionizing radiation and target analysis were used to determine its functional size in situ. Monoexponential radiation inactivation curves were obtained which indicated that a single-sized unit of 72 +/- 4 kDa is required for expression of activity. The size corresponds only to the protein portion of the target and may represent one or several polypeptides.

• OSTI ID: 6992798
• Journal Information: Journal of Lipid Research; (USA), Journal Name: Journal of Lipid Research; (USA) Vol. 30:11; ISSN JLPRA; ISSN 0022-2275
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOSYNTHESIS
BODY
CELL CONSTITUENTS
CHEMICAL RADIATION EFFECTS
CHEMISTRY
DIGESTIVE SYSTEM
ENDOPLASMIC RETICULUM
ENZYME ACTIVITY
ENZYMES
ESTERS
GENES
GLANDS
IONIZING RADIATIONS
LIPIDS
LIVER
MAMMALS
MICROSOMES
MOLECULAR WEIGHT
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
RATS
RIBOSOMES
RODENTS
SIZE
SYNTHESIS
TRANSFERASES
TRIGLYCERIDES
VERTEBRATES