ADP-ribosylation of membrane components by pertussis and cholera toxin

Journal Article · Mon Dec 31 23:00:00 EST 1984 · Methods Enzymol.; (United States)

DOI:https://doi.org/10.1016/0076-6879(85)09115-7· OSTI ID:6936308

Ribeiro-Neto, F A.P.; Mattera, F; Hildebrandt, J D; Codina, J; Field, J B; Birnbaumer, L; Sekura, R D

Pertussis and cholera toxins are important tools to investigate functional and structural aspects of the stimulatory (N/sub s/) and inhibitory (N/sub i/) regulatory components of adenylyl cyclase. Cholera toxin acts on N/sub s/ by ADP-ribosylating its ..cap alpha../sub s/ subunit; pertussis toxin acts on N/sub i/ by ADP-ribosylating its ..cap alpha..; subunit. By using (/sup 32/P)NAD/sup +/ and determining the transfer of its (/sup 32/P)ADP-ribose moiety to membrane components, it is possible to obtain information on N/sub s/ and N/sub i/. A set of protocols is presented that can be used to study simultaneously and comparatively the susceptibility of N/sub s/ and N/sub i/ to be ADP-ribosylated by cholera and pertussis toxin.

• OSTI ID: 6936308
• Journal Information: Methods Enzymol.; (United States), Journal Name: Methods Enzymol.; (United States) Vol. 109; ISSN MENZA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADP
ALDEHYDES
ANTIGENS
BACTERIAL DISEASES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CARBOHYDRATES
CELL CONSTITUENTS
CELL MEMBRANES
CHOLERA
COENZYMES
CYCLASES
DATA
DAYS LIVING RADIOISOTOPES
DISEASES
ENZYME ACTIVITY
ENZYMES
EXPERIMENTAL DATA
INFECTIOUS DISEASES
INFORMATION
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGHT NUCLEI
LYASES
MATERIALS
MEMBRANES
MONOSACCHARIDES
NAD
NUCLEI
NUCLEOTIDES
NUMERICAL DATA
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PENTOSES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOISOTOPES
REACTION KINETICS
RIBOSE
SACCHARIDES
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES