Cholera toxin can catalyze ADP-ribosylation of cytoskeletal proteins
Journal Article
·
· J. Cell Biol.; (United States)
Cholera toxin catalyzes transfer of radiolabel from (/sup 32/P)NAD/sup +/ to several peptides in particulate preparations of human foreskin fibroblasts. Resolution of these peptides by two-dimensional gel electrophoresis allowed identification of two peptides of M/sub r/ = 42,000 and 52,000 as peptide subunits of a regulatory component of adenylate cyclase. The radiolabeling of another group of peptides (M/sub r/ = 50,000 to 65,000) suggested that cholera toxin could catalyze ADP-ribosylation of cytoskeletal proteins. This suggestion was confirmed by showing that incubation with cholera toxin and (/sup 32/P)NAD/sup +/ caused radiolabeling of purified microtubule and intermediate filament proteins.
- Research Organization:
- Univ. of California, San Francisco
- OSTI ID:
- 5842828
- Journal Information:
- J. Cell Biol.; (United States), Journal Name: J. Cell Biol.; (United States) Vol. 91:2; ISSN JCLBA
- Country of Publication:
- United States
- Language:
- English
Similar Records
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Conference
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Thu May 01 00:00:00 EDT 1986
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OSTI ID:5326819
ADP-ribosylation of membrane components by pertussis and cholera toxin
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· Methods Enzymol.; (United States)
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OSTI ID:6400698
Related Subjects
550201 -- Biochemistry-- Tracer Techniques
550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADP
ANIMAL CELLS
ANTIGENS
BACTERIAL DISEASES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BODY
CATALYSIS
CHEMISTRY
CHOLERA
COENZYMES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
ENZYMES
FIBROBLASTS
INFECTIOUS DISEASES
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LIGHT NUCLEI
MATERIALS
NAD
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
SKIN
SOMATIC CELLS
TOXIC MATERIALS
TOXINS
550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADP
ANIMAL CELLS
ANTIGENS
BACTERIAL DISEASES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BODY
CATALYSIS
CHEMISTRY
CHOLERA
COENZYMES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
ENZYMES
FIBROBLASTS
INFECTIOUS DISEASES
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LIGHT NUCLEI
MATERIALS
NAD
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
SKIN
SOMATIC CELLS
TOXIC MATERIALS
TOXINS