Similarities between human eosinophil-derived neurotoxin and eosinophil cationic protein: homology with ribonuclease

Conference · Wed Mar 05 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6936237

Gleich, G J; Loegering, D A; Bell, M P; Checkel, J L; Ackerman, S J; McKean, D J

The eosinophil granule contains a series of basic proteins, including eosinophil-derived neurotoxin (EDN) and eosinophil cationic protein (ECP). EDN and ECP were isolated from eosinophil granules by heparin-Sepharose chromatography. Radioimmunoassay of fractions from heparin-Sepharose showed one peak of EDN activity and two peaks of ECP activity (termed ECP-1 and ECP-2). EDN, ECP-1, and ECP-2 each exhibited heterogeneity in charge and molecular weight when analyzed by two-dimensional electrophoresis. Digestion of EDN and both ECP's with endoglycosidase-F decreased their molecular weights indicating that their heterogeneity is due in part to n-linked oligosaccharides. Amino acid sequence analyses showed that ECP-1 and ECP-2 were identical from residues 1 through 59 and that EDN and ECP sequences were highly homologous (37 of 55 residues identical). Both EDN and ECP NH/sub 2/-terminal sequences showed significant homology to RNase, especially in regions of the RNase molecule involved in ligand binding. EDN, ECP-1 and ECP-2 had neurotoxic activity, causing the Gordon phenomenon at doses down 0.15 microgram; the proteins were comparable in their activities. These results indicate that EDN and ECP are related proteins and suggest that they derived from genes associated with the RNase family.

• Research Organization: Mayo Clinic and Foundation, Rochester, MN
• OSTI ID: 6936237
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
ANTIGENS
CHROMATOGRAPHY
COMPARATIVE EVALUATIONS
ELECTROPHORESIS
ENZYMES
ESTERASES
HYDROLASES
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
MAMMALS
MAN
MATERIALS
MOLECULAR STRUCTURE
MOLECULAR WEIGHT
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PRIMATES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RNA-ASE
SEPARATION PROCESSES
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
VERTEBRATES