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Phospholipase A/sub 2/ stimulation during cell secretion in rat basophilic leukemia cells

Journal Article · · J. Immunol.; (United States)
OSTI ID:6935859
;

The bridging of IgE receptors on rat basophilic leukemia cells (RBL-2H3) results in a number of biochemical events that accompany histamine secretion. Prominent among these is the release of arachidonic acid from cellular phospholipids, which could be due to the activation of phospholipase enzymes. In the present experiments they studied the intracellular activation of phospholipase A/sub 2/ (PLA/sub 2/) during histamine release. The enzyme in the homogenates was capable of cleaving arachidonic acid from different phospholipids. The production of lysophospholipids could play a critical role in histamine release from cells. These results demonstrate the activation of PLA/sub 2/ enzyme in cellular homogenates during the secretory process.

Research Organization:
National Institutes of Health, Bethesda, MD
OSTI ID:
6935859
Journal Information:
J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 136:1; ISSN JOIMA
Country of Publication:
United States
Language:
English

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Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
AMINES
ANIMALS
ARACHIDONIC ACID
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBON 14
CARBON ISOTOPES
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CELL CULTURES
CHEMICAL ANALYSIS
DISEASES
ENZYMES
ESTERASES
ESTERS
EVEN-EVEN NUCLEI
GLOBULINS
HEMIC DISEASES
HETEROCYCLIC COMPOUNDS
HISTAMINE
HYDROLASES
IMIDAZOLES
IMMUNOGLOBULINS
ISOTOPES
LABELLED COMPOUNDS
LEUKEMIA
LIGHT NUCLEI
LIPASE
LIPIDS
MAMMALS
MEMBRANE PROTEINS
MONOCARBOXYLIC ACIDS
NEOPLASMS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOHYDROLASES
PHOSPHOLIPIDS
PROTEINS
QUANTITATIVE CHEMICAL ANALYSIS
RADIO-RELEASE ANALYSIS
RADIOISOTOPES
RATS
RECEPTORS
RODENTS
SECRETION
VERTEBRATES
YEARS LIVING RADIOISOTOPES