Variants of 3T3 cells lacking mitogenic response to epidermal growth factor

Journal Article · Thu Sep 01 00:00:00 EDT 1977 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.74.9.3918· OSTI ID:6934942

Pruss, R M; Herschman, H R

We have previously demonstrated that epidermal growth factor (EGF) can serve as a potent mitogen for 3T3 cells. We have now selected variant 3T3 cell lines unable to respond to EGF, in order to define cellular events unique to the EGF response and to distinguish which of these events are necessary and which are merely correlative to mitogenesis. By simultaneously treating cells with EGF and colchicine, we eliminated those cells stimulated by EGF to enter mitosis. Of the eight clonal EGF nonresponder variants selected by this procedure, none retains a functional EGF receptor. The EGF nonresponsive variant lines still retain the ability to respond to other mitogens.

• Research Organization: Univ. of California, Los Angeles, CA (United States)
• OSTI ID: 6934942
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 74:9
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
MITOSIS
COLCHICINE
BIOLOGICAL EFFECTS
POLYPEPTIDES
EPIDERMIS
ALKALOIDS
ANTIMITOTIC DRUGS
BODY
CELL DIVISION
DRUGS
EPITHELIUM
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PROTEINS
SKIN
TISSUES
550300* - Cytology