Variants of 3T3 cells lacking mitogenic response to the tumor promoter tetradecanoyl-phorbol-acetate
The potent tumor promoter 12-0-tetradecanoyl-phorbol-13-acetate (TPA) can stimulate quiescent, nonproliferating 3T3 cells to reenter the cell cycle and divide. We have previously used a selection technique developed in our laboratory to isolate variant cell lines which no longer divide in response to epidermal growth factor. We have now utilized the same selection procedure to isolate, from 3T3 cells, two variant cell lines, TNR-2 and TNR-9, which retain growth control and divide in response to elevated serum or fibroblast growth factor, but which do not respond to TPA. The variants do not incorporate precursors into DNA in response to TPA, demonstrating that the cells do not enter the S phase of the cell cycle. The TPA nonresponsive variant TNR-2 cannot respond to epidermal growth factor; TNR-9 responds to this mitogen. TNR-2 variant cells, which do not respond to EGF, do not bind /sup 125/I-EGF.
- Research Organization:
- Univ. of California, Los Angeles
- DOE Contract Number:
- AM03-76-SF00012
- OSTI ID:
- 6503160
- Journal Information:
- J. Cell. Physiol.; (United States), Vol. 107:1
- Country of Publication:
- United States
- Language:
- English
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TUMOR PROMOTERS
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550301* - Cytology- Tracer Techniques