Phanerochaete mutants with enhanced ligninolytic activity

Journal Article · · Applied Biochemistry and Biotechnology; (United States)

DOI:https://doi.org/10.1007/BF02941810· OSTI ID:6929306

Kakar, S N; Perez, A; Gonzales, J ^[1]

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1. Argonne National Lab., IL (United States)

In addition to lignin, the white rot fungus Phanerochaete chrysosporium has the ability to degrade a wide spectrum of recalcitrant organopollutants in soils and aqueous media. Most of the organic compounds are degraded under ligninolytic conditions with the involvement of the extracellular enzymes, lignin peroxidases, and manganese-dependent peroxidases, which are produced as secondary metabolites triggered by conditions of nutrient starvation (e.g., nitrogen limitation). The fungus and its enzymes can thus provide alternative technologies for bioremediation, biopulping, biobleaching, and other industrial applications. The efficiency and effectiveness of the fungus can be enhanced by increasing production and secretion of the important enzymes in large quantities and as primary metabolites under enriched conditions. One way this can be achieved is through isolation of mutants that are deregulated, or are hyperproducers or supersectors of key enzymes under enriched conditions. Through UV-light and [gamma]-ray mutagenesis, the authors have isolated a variety of mutants, some of which produce key enzymes of the ligninolytic system under high-nitrogen growth conditions. One of the mutants, 76UV, produced 272 U of lignin peroxidases enzyme activity/L after 9 d under high nitrogen (although the parent strain does not produce this enzyme under these conditions). The mutant and the parent strains produced up to 54 and 62 U/L, respectively, of the enzyme activity under low-nitrogen growth conditions during this period. In some experiments, the mutant showed 281 U/L of enzyme activity under high nitrogen after 17 d. 17 refs., 1 fig., 3 tabs.

• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 6929306
• Journal Information: Applied Biochemistry and Biotechnology; (United States), Journal Name: Applied Biochemistry and Biotechnology; (United States) Vol. 45-46; ISSN 0273-2289; ISSN ABIBDL
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
090900* -- Biomass Fuels-- Processing-- (1990-)
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
EUMYCOTA
FUNGI
HYDROLYSIS
LIGNIN
LYSIS
MUTATIONS
ORGANIC COMPOUNDS
PHANEROCHAETE
PLANTS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS