Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

DNA repair after gamma radiation and superoxide dismutase activity in lymphocytes from subjects of far advanced age

Journal Article · · Carcinogenesis (N.Y.); (United States)
; ; ; ; ; ; ;
DNA repair after gamma radiation was studied in purified T lymphocytes from young and aged subjects. Two different assays were employed. In the first, T lymphocytes were stimulated with phytohemagglutinin (PHA) for 72 h and then treated with hydroxyurea, irradiated with 30 K rads and pulsed with (/sup 3/H)thymidine (TdR) for 4 h. In the second, T lymphocytes were first irradiated with graded doses of gamma rays (200-800 rads) and then stimulated with PHA, cultured for 72 h and pulsed with /sup 3/H-TdR for the last 6 h of culture. T lymphocytes from aged subjects showed a lack of DNA repair synthesis in the first assay whereas only minor differences were found in the second assay between the two groups. Lymphocyte superoxide dismutase activity showed great individual variations in both groups and a slight increase in old subjects.
Research Organization:
Institute of General Pathology, University of Bologna, Bologna, Italy
OSTI ID:
6929158
Journal Information:
Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 3:1; ISSN CRNGD
Country of Publication:
United States
Language:
English

Similar Records

DNA repair after gamma radiation and superoxide dismutase activity in lymphocytes from subjects of far advanced age
Journal Article · Thu Dec 31 23:00:00 EST 1981 · Carcinogenesis (N.Y.); (United States) · OSTI ID:5132442
DNA repair after gamma irradiation in lymphocytes exposed to low-frequency pulsed electromagnetic fields
Journal Article · Fri Mar 31 23:00:00 EST 1989 · Radiat. Res.; (United States) · OSTI ID:6253591
Sensitivity of cultured lymphocytes from patients with nevoid basal cell carcinoma syndrome to ultraviolet light and phytohemagglutinin stimulation
Journal Article · Sun Dec 31 23:00:00 EST 1989 · Teratogenesis, Carcinogenesis, and Mutagenesis; (United States) · OSTI ID:5506530

Related Subjects

550401 -- Genetics-- Tracer Techniques
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGGLUTININS
AMIDES
ANIMAL CELLS
ANTIBODIES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARBONIC ACID DERIVATIVES
CELL CULTURES
COMPARATIVE EVALUATIONS
CONNECTIVE TISSUE CELLS
DNA
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
GAMMA RADIATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HEMAGGLUTININS
IONIZING RADIATIONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MITOGENS
MUCOPROTEINS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHYTOHEMAGGLUTININ
POLYSACCHARIDES
PROTEINS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SACCHARIDES
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UREA