DNA repair after gamma irradiation in lymphocytes exposed to low-frequency pulsed electromagnetic fields

Journal Article · Sat Apr 01 04:00:00 EST 1989 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3577431· OSTI ID:6253591

Cossarizza, A; Monti, D; Sola, P; Moschini, G; Cadossi, R; Bersani, F; Franceschi, C

The effect of exposure to extremely low-frequency pulsed electromagnetic fields (EMFs) on DNA repair capability and on cell survival in human lymphocytes damaged in vitro with gamma rays was studied by two different micromethods. In the first assay, which measures DNA repair synthesis (unscheduled DNA synthesis, UDS), lymphocyte cultures were stimulated with phytohemagglutinin (PHA) for 66 h and then treated with hydroxyurea (which blocks DNA replication), irradiated with 100 Gy of /sup 60/Co, pulsed with (/sup 3/H)thymidine ((/sup 3/H)TdR), and then exposed to pulsed EMFs for 6 h (the period in which cells repaired DNA damage). In the second assay, which measures cell survival after radiation or chemical damage, lymphocytes were first irradiated with graded doses of gamma rays or treated with diverse antiproliferative agents, and then stimulated with PHA, cultured for 72 h, and pulsed with (/sup 3/H)TdR for the last 6 h of culture. In this case, immediately after the damage induced by either the radiation or chemicals, cultures were exposed to pulsed EMFs for 72 h, during which cell proliferation took place. Exposure to pulsed EMFs did not affect either UDS or cell survival, suggesting that this type of nonionizing radiation--to which humans may be exposed in the environment, and which is used for both diagnostic and therapeutic purposes--does not affect DNA repair mechanisms.

• Research Organization: Univ. of Modena (Italy)
• OSTI ID: 6253591
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 118:1; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

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560120* -- Radiation Effects on Biochemicals
Cells
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560400 -- Other Environmental Pollutant Effects
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGGLUTININS
AMIDES
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIBODIES
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BLEOMYCIN
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
COBALT 60
COBALT ISOTOPES
CONNECTIVE TISSUE CELLS
DNA
DNA REPAIR
DOXORUBICIN
DRUGS
ELECTROMAGNETIC FIELDS
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HEMAGGLUTININS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
HYDROXYUREA
IN VITRO
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MINUTES LIVING RADIOISOTOPES
MITOGENS
MUCOPROTEINS
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHYTOHEMAGGLUTININ
POLYSACCHARIDES
PRIMATES
PROTEINS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RECOVERY
REPAIR
RIBOSIDES
SACCHARIDES
SOMATIC CELLS
THYMIDINE
TRACER TECHNIQUES
VERTEBRATES
YEARS LIVING RADIOISOTOPES