Estrogen suppression of I-125-cholesteryl ester uptake in atherosclerotic lesions in a rabbit model

Conference · Sun Jan 01 04:00:00 EST 1984 · J. Nucl. Med.; (United States)

OSTI ID:6929028

De Galen, M R; Schwender, S W; Skinner, R W.S.; Gross, M D; Counsell, R E

Cholesteryl esters (CE) are the principal lipids found in atherosclerotic lesions. A nonhydrolyzable CE analog, I-125-cholesteryl iopanoate (I-125-CI), that is rapidly associated with circulating lipoproteins was administered to New Zealand White rabbits rendered hypercholesterolemic to assess the effect of hypercholesterolemia and estrogen therapy upon the uptake of CI into developing atherosclerotic lesions. Atherosclerosis (A) was induced by aortic deendothelialization in 10 rabbits with a balloon embolectomy catheter introduced via the femoral artery, followed by a 2% cholesterol-enriched diet. Five of these 10 rabbits were treated with estradiol (AE) (0.5mg/Kg/week x 5); an additional 4 animals served as untreated, non-A controls. I-125-CI was administered intravenously in multiple doses over a 5 week interval. Gamma camera images and selected tissues were obtained 6 days after the completion of I-125-CI administration (total cumulative dose 82.6-87.3..mu..Ci/rabbit). Plasma cholesterol levels were 268 +- 162, 1940 +- 248 and 2484 +- 209 mg/dl in the untreated controls, A and AE groups, respectively. Atherosclerotic aortas were readily visualized in the A > AE groups as compared to untreated controls. Abdominal aortic tissues contained 0.013 +- 0.004, 0.187 +- 0.021, and 0.067 +- 0.015 %-Kg/dose/gm of I-125-CI from the untreated controls, A and AE groups, respectively. Thus, CI analogs can be used as probes to scintigraphically depict CE distribution and the effects of pharmacologic manipulation upon the accumulation of CE by vascular lesions in an animal model of atherosclerosis.

• Research Organization: Univ. of Michigan VA Medical Centers, Ann Arbor, MI 48105
• OSTI ID: 6929028
• Report Number(s): CONF-840619-
• Conference Information: Journal Name: J. Nucl. Med.; (United States) Journal Volume: 25:5
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
AORTA
ARTERIES
ARTERIOSCLEROSIS
BETA DECAY RADIOISOTOPES
BLOOD CHEMISTRY
BLOOD VESSELS
BODY
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CHOLESTEROL
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DIAGNOSTIC TECHNIQUES
DISEASES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ESTERS
ESTRADIOL
ESTRANES
ESTROGENS
HORMONES
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LIPIDS
LIPOPROTEINS
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PROTEINS
RABBITS
RADIOISOTOPE SCANNING
RADIOISOTOPES
RADIOPHARMACEUTICALS
SCINTISCANNING
STEROID HORMONES
STEROIDS
STEROLS
THERAPEUTIC USES
UPTAKE
USES
VASCULAR DISEASES
VERTEBRATES