Detergent micelle structure and micelle-micelle interactions determined by small-angle neutron scattering under solution conditions used for membrane protein crystallization
- Argonne National Lab., IL (United States)
We have characterized micelle structure and intermicelle interaction for the detergents lauryldimethylamine N-oxide, LDAO, and n-octyl-[beta]-D-glucoside, OG, under conditions used for protein crystallization using SANS. We found that LDAO and OG micelles differ significantly in size, sensitivity to heptanetriol, and nature of intermicelle interactions. Our results suggest that successful crystallization methods can be rationalized in terms of an optimization of micelle size, number density, flexibility of micelle radius of curvature, and suppression of intermicelle interactions. LDAO and OG micelles were found to differ significantly in size and shape. The LDAO micelle was found to be best fit as an ellipsoid with semiaxes of 30.6 and 19.4 [angstrom], while the OG micelle was found to be spherical with a radius of 22.9 [angstrom]. The addition of heptanetriol to pure LDAO resulted in the formation of smaller, spherical, mixed micelles with radii in the range 17-21 [angstrom], depending upon conditions. The results suggest that both micelle size and curvature restrictions may contribute to the incompatibility of LDAO for protein crystallization in the absence of additional amphiphiles. 32 refs., 8 figs., 3 tabs.
- DOE Contract Number:
- W-31109-ENG-38
- OSTI ID:
- 6928995
- Journal Information:
- Journal of Physical Chemistry; (United States), Vol. 98:40; ISSN 0022-3654
- Country of Publication:
- United States
- Language:
- English
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HEAVY WATER
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EMULSIFIERS
FERMIONS
GLYCOLS
HADRONS
HALIDES
HALOGEN COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INFORMATION
MICROORGANISMS
NUCLEONS
NUMERICAL DATA
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ORGANIC POLYMERS
OXYGEN COMPOUNDS
PHASE TRANSFORMATIONS
POLYMERS
PROTEINS
SODIUM COMPOUNDS
SULFATES
SULFUR COMPOUNDS
SURFACTANTS
WATER
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