Submicrometer Emitter ESI Tips for Native Mass Spectrometry of Membrane Proteins in Ionic and Nonionic Detergents

Journal Article · Mon Jan 15 04:00:00 EST 2018 · Journal of the American Society for Mass Spectrometry

DOI:https://doi.org/10.1007/S13361-017-1793-X· OSTI ID:22776813

Susa, Anna C. ^[1]; Lippens, Jennifer L. ^[2]; Xia, Zijie ^[1]; Loo, Joseph A. ^[3]; Campuzano, Iain D. G., ^[2]

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1. University of California, Department of Chemistry (United States)
2. Discovery Analytical Sciences, Amgen (United States)
3. University of California-Los Angeles, Department of Chemistry and Biochemistry, and Department of Biological Chemistry (United States)

Native mass spectrometry (native-MS) of membrane proteins typically requires a detergent screening protocol, protein solubilization in the preferred detergent, followed by protein liberation from the micelle by collisional activation. Here, submicrometer nano-ESI emitter tips are used for native-MS of membrane proteins solubilized in both nonionic and ionic detergent solutions. With the submicrometer nano-ESI emitter tips, resolved charge-state distributions of membrane protein ions are obtained from a 150 mM NaCl, 25 mM Tris-HCl with 1.1% octyl glucoside solution. The relative abundances of NaCl and detergent cluster ions at high m /z are significantly reduced with the submicrometer emitters compared with larger nano-ESI emitters that are commonly used. This technique is beneficial for significantly decreasing the abundances (by two to three orders of magnitude compared with the larger tip size: 1.6 μm) of detergent cluster ions formed from aqueous ammonium acetate solutions containing detergents that can overlap with the membrane protein ion signal. Resolved charge-state distributions of membrane protein ions from aqueous ammonium acetate solutions containing ionic detergents were obtained with the submicrometer nano-ESI emitters; this is the first report of native-MS of membrane proteins solubilized by ionic detergents. .

• OSTI ID: 22776813
• Journal Information: Journal of the American Society for Mass Spectrometry, Journal Name: Journal of the American Society for Mass Spectrometry Journal Issue: 1 Vol. 29; ISSN 1044-0305; ISSN JAMSEF
• Country of Publication: United States
• Language: English

Related Subjects

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY
CHARGE STATES
COMPARATIVE EVALUATIONS
ION PAIRS
MASS SPECTROSCOPY
MEMBRANE PROTEINS
SIGNALS
SOLUTIONS