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Distribution and identity of the earliest proliferating progeny of colony-forming cells in regenerating murine spleen and bone marrow

Journal Article · · Am. J. Anat.; (United States)
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A study was made of the sites of development and the types of cells found in very early hemopoietic colonies in the mouse spleen. Two, 3, and 4 days after transplantation, the proliferating descendants of transplanted bone-marrow cells were identified on radioautographs of spleen sections and on spleen and bone-marrow smears of supralethally irradiated recipient mice which were injected with /sup 3/H-TdR at 12, 6, and 0.5 hours before sacrifice. Surprisingly the spleens of nontransplanted, irradiated mice contained proliferating medium and large lymphocytes in the white pulp which increased in numbers during the observation period. The early descendants of transplanted cells that lodged in the spleen could be clearly distinguished from the labeled indigenous cells because they formed discrete nodules or colonies beneath the splenic capsule or in the vicinity of venules and trabeculae of the red pulp. These cells were identifiable on day 2 as transitional cells or unknown hemopoietic blasts and on day 4 included early erythroid cells and small lymphocytes. There was evidence for the traffic of /sup 3/H-TdR-labeled cells through the splenic sinusoids.

Research Organization:
Department of Pathology, University of Washington, Seattle
OSTI ID:
6928965
Journal Information:
Am. J. Anat.; (United States), Journal Name: Am. J. Anat.; (United States) Vol. 163:2; ISSN AJANA
Country of Publication:
United States
Language:
English

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Related Subjects

550201 -- Biochemistry-- Tracer Techniques
550301 -- Cytology-- Tracer Techniques
560151* -- Radiation Effects on Animals-- Man
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BODY
BONE MARROW
BONE MARROW CELLS
CELL DIFFERENTIATION
CELL DIVISION
COLONY FORMATION
CONNECTIVE TISSUE CELLS
HEMATOPOIETIC SYSTEM
IRRADIATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MICE
ORGANS
PHYSIOLOGY
RODENTS
SOMATIC CELLS
SPLEEN
SPLEEN CELLS
SPLEEN COLONY FORMATION
SUPRALETHAL IRRADIATION
TISSUES
TRITIUM COMPOUNDS
VERTEBRATES