Comparative studies on the characteristics of proliferation and differentiation of spleen colony-forming cells

Journal Article · Fri Nov 01 04:00:00 EST 1985 · Int. J. Cell Cloning; (United States)

OSTI ID:5737742

Wu, C T; Liu, M P; Chu, J P

Using a single spleen colony transplantation technique and sex chromosome typing as a natural cytogenetic marker, most spleen colony-forming cells (CFC) in adult bone marrow or fetal livers of inbred LACA or C57 mice re-established hemopoiesis in lethally irradiated mice when the spleen colonies were sampled at 13 days after transplantation. However, most of the spleen colony-forming cells in the peripheral blood of normal mice possess little potential for proliferation and are less efficient in the re-establishment of hemopoiesis in lethally irradiated mice. The CFC population is heterogeneous in the mice. From the subsequent retransplantation of colonies from colony-forming cells in the peripheral blood, the simple assessment of spleen colony-forming units (CFU-s) content, based on the number of splenic colonies, does not reliably represent the content of hemopoietic stem cells.

• OSTI ID: 5737742
• Journal Information: Int. J. Cell Cloning; (United States), Journal Name: Int. J. Cell Cloning; (United States) Vol. 6; ISSN IJCCE
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BLOOD FORMATION
BODY
BONE MARROW
CELL DIFFERENTIATION
CELL PROLIFERATION
CFU
COLONY FORMATION
DIGESTIVE SYSTEM
GLANDS
HEMATOPOIETIC SYSTEM
IRRADIATION
LETHAL IRRADIATION
LIVER
MAMMALS
MICE
ORGANS
RADIATION EFFECTS
RODENTS
SPLEEN
SPLEEN COLONY FORMATION
TISSUES
VERTEBRATES