Dimer excision in Escherichia coli in the presence of caffeine

Journal Article · Tue Jul 01 00:00:00 EDT 1980 · J. Bacteriol.; (United States)

OSTI ID:6926528

Rothman, R H

The observation that polA1 and recL152 mutations result in both slow pyrimidine dimer excision and large repair patch size leads to the hypothesis that patch size is directly related to the rate of excision. In this study caffeine, a known inhibitor of excision repair, was used to examine the extent of correlation between excision rate and patch size by measuring patch size in the presence of several concentrations of caffeine. Both the rate of excision and the resistance to ultraviolet radiation were reduced with increasing concentrations of caffeine after irradiation. Caffeine also inhibited the rate at which incisions were made and prolonged the time required to rejoin the discontinuities. Patch size, however, was unaffected by caffeine treatment.

• Research Organization: Brookhaven National Lab., Upton, NY
• OSTI ID: 6926528
• Journal Information: J. Bacteriol.; (United States), Vol. 143:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CAFFEINE
BIOLOGICAL EFFECTS
DIMERS
ANIMAL CELLS
BIOLOGICAL REPAIR
ESCHERICHIA COLI
GROWTH
INHIBITION
MUTANTS
PYRIMIDINES
ULTRAVIOLET RADIATION
AZINES
BACTERIA
BIOLOGICAL RECOVERY
DRUGS
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PURINES
RADIATIONS
RECOVERY
REPAIR
XANTHINES
550200* - Biochemistry
550700 - Microbiology
550400 - Genetics