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Human plasma lecithin-cholesterol acyltransferase

Journal Article · · J. Biol. Chem.; (United States)
OSTI ID:6925213
; ;

Lecithin-cholesterol acyltransferase (LCAT) is a plasma enzyme which catalyzes the transacylation of the fatty acid at the sn-2 position of lecithin to cholesterol forming lysolecithin and cholesteryl ester. The substrates for and products of this reaction are present within the plasma lipoproteins upon which the enzyme acts to form the majority of cholesteryl ester in human plasma. The authors proposed a covalent catalytic mechanism of action for LCAT in which serine and histidine residues mediate lecithin cleavage and two cysteine residues cholesterol esterification. With the aid of sulfhydryl reactive trivalent organoarsenical compounds which are specific for vicinal thiols they have probed the geometry of the catalytic site. They conclude that the two catalytic cysteine residues of LCAT (Cys/sup 31/ and Cys /sup 184/) are vicinal with a calculated distance between their sulfur atoms of 3.50-3.62 A. The additional residue alkylated by teh bifunctional reagent is within the catalytic site and may represent a previously identified catalytic serine or histidine residue.

Research Organization:
Dalhousie Univ., Halifax, Nova Scotia (Canada)
OSTI ID:
6925213
Journal Information:
J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 263:14; ISSN JBCHA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACYLATION
AMINO ACIDS
ANIMALS
AZOLES
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD PLASMA
BODY FLUIDS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHEMISTRY
CHOLESTEROL
CYSTEINE
ENZYMES
ESTERS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HISTIDINE
HYDROXY ACIDS
HYDROXY COMPOUNDS
IMIDAZOLES
KINETICS
LABELLED COMPOUNDS
LECITHINS
LIPIDS
LIPOPROTEINS
MAMMALS
MAN
MATERIALS
MOLECULAR STRUCTURE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHOLIPIDS
PRIMATES
PROTEINS
REACTION KINETICS
SERINE
STEROIDS
STEROLS
SUBSTRATES
SYNTHESIS
THIOLS
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES