Metabolism of low-density lipoprotein free cholesterol by human plasma lecithin-cholesterol acyltransferase

Journal Article · Tue Sep 03 04:00:00 EDT 1991 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00099a009· OSTI ID:5820226

Fielding, P E; Miida, Takashi; Fielding, C J ^[1]

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1. Univ. of California, San Francisco (United States)

The metabolism of cholesterol derived from ({sup 3}H) cholesterol-labeled low-density lipoprotein (LDL) was determined in human blood plasma. LDL-derived free cholesterol first appeared in large {alpha}-migrating HDL (HDL{sub 2}) and was then transferred to small {alpha}-HDL (HDL{sub 3}) for esterification. The major part of such esters was retained within HDL of increasing size in the course of lecithin-cholesterol acyltransferase (LCAT) activity; the balance was recovered in LDL. Transfer of preformed cholesteryl esters within HDL contributed little to the labeled cholesteryl ester accumulating HDL{sub 2}. When cholesterol for esterification was derived instead from cell membranes, a significantly smaller proportion of this cholesteryl ester was subsequently recovered in LDL. These data suggest compartmentation of cholesteryl esters within plasma that have been formed from cell membrane or LDL free cholesterol, and the role for HDL{sub 2} as a relatively unreactive sink for LCAT-derived cholesteryl esters.

• OSTI ID: 5820226
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 30:35; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550600* -- Medicine
62 RADIOLOGY AND NUCLEAR MEDICINE
AUTORADIOGRAPHY
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY FLUIDS
CHEMICAL REACTIONS
CHOLESTEROL
COMPARTMENTS
ENZYMES
ESTERIFICATION
GLYCOSYL TRANSFERASES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
LIPIDS
LIPOPROTEINS
MATERIALS
METABOLISM
ORGANIC COMPOUNDS
PROTEINS
STEROIDS
STEROLS
TRANSFERASES
TRITIUM COMPOUNDS