Identification of hormone-interacting amino acid residues within the steroid-binding domain of the glucocorticoid receptor in relation to other steroid hormone receptors

Journal Article · Sun May 15 04:00:00 EDT 1988 · J. Biol. Chem.; (United States)

OSTI ID:6925125

Carlstedt-Duke, J; Stroemstedt, P E; Persson, B; Cederlund, E; Gustafsson, J A; Joernvall, H

Purified rat liver glucocorticoid receptor was covalently charged with (/sup 3/H)glucocorticoid by photoaffinity labeling (UV irradiation of (/sup 3/H)triamcinolone acetonide-glucocorticoid receptor) or affinity labeling (incubation with (/sup 3/H)dexamethasone mesylate). After labeling, separate samples of the denatured receptor were cleaved with trypsin (directly or after prior succinylation), chymotrypsin, and cyanogen bromide. Labeled residues in the peptides obtained were identified by radiosequence analysis. The peaks of radioactivity corresponded to Met-622 and Cys-754 after photoaffinity labeling with (/sup 3/H)triamcinolone acetonide and Cys-656 after affinity labeling with (/sup 3/H)dexamethasone mesylate. The labeled residues are all positioned within hydrophobic segments of the steroid-binding domain. The patterns of hydropathy and secondary structure for the glucocorticoid receptor are highly similar to those for the progestin receptor and similar but less so to those for the estrogen receptor and to those for c-erb A.

• Research Organization: Karolinska Institutet, Huddinge (Sweden)
• OSTI ID: 6925125
• Journal Information: J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 263:14; ISSN JBCHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
AMINO ACID SEQUENCE
ANIMALS
BODY
CHEMISTRY
CONFIGURATION INTERACTION
CORTICOSTEROIDS
DIGESTIVE SYSTEM
DNA
ELECTROMAGNETIC RADIATION
EXTREME ULTRAVIOLET RADIATION
GLANDS
GLUCOCORTICOIDS
HYDROXY COMPOUNDS
KETONES
LABELLED COMPOUNDS
LIGANDS
LIVER
MAMMALS
MEMBRANE PROTEINS
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PREGNANES
PROTEINS
RADIATIONS
RATS
RECEPTORS
RODENTS
STEROIDS
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
VERTEBRATES