Radiosequence analysis of the human progestin receptor charged with ( sup 3 H)promegestone. A comparison with the glucocorticoid receptor

Journal Article · Sun Aug 05 04:00:00 EDT 1990 · Journal of Biological Chemistry; (USA)

OSTI ID:6795497

Stroemstedt, P E.B.; Berkenstam, A; Joernvall, H G; Gustafsson, J A; Carlstedt-Duke, J ^[1]

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1. Huddinge Univ. Hospital (Sweden)

Partially purified preparations of the human progestin receptor and the human and rat glucocorticoid receptor proteins were covalently charged with the synthetic progestin, ({sup 3}H)promegestone, by photoaffinity labeling. After labeling, the denaturated protein was cleaved and the mixture of peptides subjected to radiosequence analysis as previously described for the rat glucocorticoid receptor protein. The radioactivity labels identified, corresponded to Met-759 and Met-909 after photoaffinity labeling of the human progestin receptor, and Met-622 and Cys-754 after labeling of the rat glucocorticoid receptor. The residues labeled in the glucocorticoid receptor are the same as those previously reported to bind triamcinolone actonide. The corresponding residues were also labeled in the human glucocorticoid receptor. Met-759 of the progestin receptor and Met-622 of the rat glucocorticoid receptor are positioned within a segment with an overall high degree of sequence similarity and are equivalent. However, Met-909 (progestin receptor) and Cys-754 (glucocorticoid receptor) do not occur within equivalent segments of the two proteins. Thus, although the two classes of steroid hormone share a common structure within the A-ring, there are subtle differences in their interaction with the two separate receptor proteins.

• OSTI ID: 6795497
• Journal Information: Journal of Biological Chemistry; (USA), Journal Name: Journal of Biological Chemistry; (USA) Vol. 265:22; ISSN 0021-9258; ISSN JBCHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
AFFINITY
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMALS
BODY
CARBOXYLIC ACIDS
COMPARATIVE EVALUATIONS
CORTICOSTEROIDS
CYSTEINE
DEXAMETHASONE
DIGESTIVE SYSTEM
DISEASES
DRUGS
GLANDS
GLUCOCORTICOIDS
HORMONES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
LABELLING
LIPOTROPIC FACTORS
LIVER
MAMMALS
MAMMARY GLANDS
MAN
MEMBRANE PROTEINS
METHIONINE
MOLECULAR STRUCTURE
NEOPLASMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PEPTIDES
PREGNANES
PRIMATES
PROGESTERONE
PROTEINS
RATS
RECEPTORS
RODENTS
STEROID HORMONES
STEROIDS
THIOLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES