Detection of CCl/sub 4/-induced oxidation of hepatic tissue in vivo by oxygen-18 tracing

Technical Report · Fri Jan 01 00:00:00 EST 1988

OSTI ID:6924615

Hatch, G E; Santrock, J; Slade, R; Hayes, J M

Oxygen can become a damaging influence in tissues and cells exposed to environmental pollutants. This paper describes the first application of a new technique for tracing oxygen in tissues exposed to pollutants. Carbon tetrachloride (CCl/sub 4/) was found to cause oxidation of liver tissue in rats which was measurable using oxygen-18 labeling procedures. Rats that breathed oxygen-18 while being exposed to CCl/sub 4/ were found to have oxidized lipids, macromolecules, and water-soluble substances in their livers. The techniques outlined in the paper should be useful for elucidating mechanisms of injury and increased aging of tissues exposed to pollutants.

• Research Organization: Environmental Protection Agency, Research Triangle Park, NC (USA). Health Effects Research Lab.
• OSTI ID: 6924615
• Report Number(s): PB-88-224381/XAB; EPA-600/J-88/041
• Resource Relation: Other Information: Pub. in Toxicology and Applied Pharmacology, Vol. 93, 81-88(1988)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
LIVER CELLS
OXIDATION
OXYGEN 18
TRACER TECHNIQUES
POLLUTANTS
BIOLOGICAL EFFECTS
CARBON TETRACHLORIDE
IN VIVO
LABORATORY ANIMALS
LIPIDS
MOLECULES
ANIMAL CELLS
CHEMICAL REACTIONS
CHLORINATED ALIPHATIC HYDROCARBONS
EVEN-EVEN NUCLEI
HALOGENATED ALIPHATIC HYDROCARBONS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
NUCLEI
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXYGEN ISOTOPES
SOMATIC CELLS
STABLE ISOTOPES
550300* - Cytology