Radioimmunolocalization of human carcinoma xenografts with B72. 3 second generation monoclonal antibodies

Journal Article · Mon Aug 15 04:00:00 EDT 1988 · Cancer Res.; (United States)

OSTI ID:6924350

Colcher, D; Minelli, M F; Roselli, M; Muraro, R; Simpson-Milenic, D; Schlom, J

The B72.3 reactive antigen, TAG-72, has been purified and a series of second generation monoclonal antibodies (MAbs), designated CC (colon cancer), have been characterized by a range of in vitro immunological assays. Six CC MAbs (CC11, CC30, CC46, CC49, CC83, and CC92) were chosen for analyses of the in vivo binding to a human colon carcinoma xenograft. All 6 MAbs were previously shown to be distinct from B72.3 and each other by a series of reciprocal competition radioimmunoassays, and all were shown to have a Ka higher than that of B72.3. In this study we demonstrate that all six CC MAbs evaluated are superior to B72.3 in an in vivo tumor targeting model, using human colon carcinoma (LS-174T) xenografts in athymic mice, in terms of both the percentage of the injected dose of radiolabeled MAb delivered per g of tumor and tumor:normal tissue ratios. Differences in the in vivo binding patterns and pharmacokinetics among the CC MAbs are also evaluated. Thus, in light of the fact that B72.3 has been shown to successfully target approximately 75% of primary and metastatic carcinoma lesions in a variety of different carcinoma types in over 300 patients, these studies serve as further evidence to support the clinical evaluation of the second generation CC MAbs, either alone or in combination with B72.3.

• Research Organization: National Cancer Institute, Bethesda, MD (USA)
• OSTI ID: 6924350
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 48:16; ISSN CNREA
• Country of Publication: United States
• Language: English

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550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
BIOLOGICAL LOCALIZATION
BIOLOGICAL MODELS
BODY
CARCINOMAS
DIGESTIVE SYSTEM
DISEASES
EXPERIMENTAL NEOPLASMS
GASTROINTESTINAL TRACT
IMMUNOASSAY
IMMUNOLOGY
INTESTINES
ISOTOPE APPLICATIONS
LARGE INTESTINE
MAMMALS
MICE
MONOCLONAL ANTIBODIES
NEOPLASMS
ORGANS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RODENTS
TRACER TECHNIQUES
TRANSPLANTS
VERTEBRATES