Thrombus detection using a Tc-99m labeled antifibrin monoclonal antibody (MoAb). Experiments in vitro and in animals

Journal Article · Mon Jan 01 04:00:00 EST 1990 · Thrombosis Research; (USA)

DOI:https://doi.org/10.1016/0049-3848(90)90383-N· OSTI ID:6920802

Wasser, M N; Pauwels, E K; Nieuwenhuizen, W ^[1]

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1. Gaubius Institute TNO, Leiden (Netherlands)

This paper describes the production and characterization of an antifibrin monoclonal antibody, produced by immunizing mice with fibrinogen degradation fragment Y. The antibody (designated Y22) is directed against a conformation dependent epitope in the D-domain of fibrin Y22 was labeled with the radionuclide Tc-99m. Experiments in vitro and in rats are presented which show the potential of scintigraphic detection of thrombi with Tc-99m-Y22. Preliminary results show that immunoscintigraphy of thrombi may also have potential for the monitoring of thrombolytic therapy (eg with t-PA).

• OSTI ID: 6920802
• Journal Information: Thrombosis Research; (USA), Journal Name: Thrombosis Research; (USA) Vol. 10; ISSN THBRA; ISSN 0049-3848
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARDIOVASCULAR DISEASES
CHEMICAL COMPOSITION
COAGULANTS
COUNTING TECHNIQUES
DIAGNOSIS
DIAGNOSTIC TECHNIQUES
DISEASES
DRUGS
FIBRIN
FLUORESCENCE
HEMATOLOGIC AGENTS
HEMOSTATICS
HOURS LIVING RADIOISOTOPES
IN VITRO
IN VIVO
INTERMEDIATE MASS NUCLEI
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LUMINESCENCE
MAMMALS
MICE
MONOCLONAL ANTIBODIES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOIMMUNODETECTION
RADIOIMMUNOSCINTIGRAPHY
RADIOISOTOPE SCANNING
RADIOISOTOPES
RATS
RODENTS
SCINTISCANNING
SCLEROPROTEINS
TECHNETIUM 99
TECHNETIUM ISOTOPES
THROMBOSIS
TRACER TECHNIQUES
VASCULAR DISEASES
VERTEBRATES
YEARS LIVING RADIOISOTOPES