An antifibrin monoclonal antibody useful in immunoscintigraphic detection of thrombi

Journal Article · Tue Aug 01 04:00:00 EDT 1989 · Blood; (USA)

OSTI ID:5604913

Wasser, M N; Koppert, P W; Arndt, J W; Emeis, J J; Feitsma, R I; Pauwels, E K; Nieuwenhuizen, W ^[1]

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1. Gaubius Institute TNO, Leiden (Netherlands)

Balb/c mice were immunized with human plasmin-generated fibrinogen degradation product Y. Spleen cells were fused with P3X63-Ag8.653 myeloma cells. A clone (Y22) was found that produces monoclonal antibodies (MoAbs) with a strong reactivity with human fibrin and only a weak reactivity with fibrinogen in an enzyme immunoassay (EIA). Y22 also reacts with fibrin of rabbits, rats, sheep, and dogs. The antibodies are of the IgG1 kappa-type and appear to be directed against a conformation-dependent epitope in the D-domain of fibrin. Experiments with {sup 99m}Tc-labeled Y22 in vitro show that Y22 binds rapidly to forming clots. {sup 99}mTc-Y22 also binds to preformed plasma clots in a plasma milieu, even in the presence of high concentrations of heparin. Clot localization experiments in rabbits and rats confirm the high fibrin specificity and the potential of {sup 99m}Tc-Y22 for thrombus imaging in vivo.

• OSTI ID: 5604913
• Journal Information: Blood; (USA), Journal Name: Blood; (USA) Vol. 74:2; ISSN BLOOA; ISSN 0006-4971
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ABDOMEN
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOASSAY
BLOOD COAGULATION FACTORS
BLOOD VESSELS
BODY
BODY AREAS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CELL CULTURES
CLONE CELLS
COAGULANTS
COUNTING TECHNIQUES
DIAGNOSIS
DIAGNOSTIC TECHNIQUES
DISEASES
DRUGS
ELEMENTS
ENZYME IMMUNOASSAY
FIBRIN
FIBRINOGEN
GLOBULINS
HEMATOLOGIC AGENTS
HEMOSTATICS
IMMUNOASSAY
ISOMERIC NUCLEI
ISOTOPE APPLICATIONS
MAMMALS
METALS
MICE
MONOCLONAL ANTIBODIES
NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RABBITS
RADIOIMMUNODETECTION
RADIOIMMUNOSCINTIGRAPHY
RADIOISOTOPE SCANNING
RATS
RODENTS
SCINTISCANNING
SCLEROPROTEINS
SOMATIC CELLS
SPECIFICITY
SPLEEN CELLS
TECHNETIUM
THROMBOSIS
TRACER TECHNIQUES
TRANSITION ELEMENTS
TUMOR CELLS
VASCULAR DISEASES
VEINS
VERTEBRATES