Formation of alpha-deoxyadenosine in polydeoxynucleotides exposed to ionizing radiation under anoxic conditions

Journal Article · Thu Mar 01 04:00:00 EST 1990 · Radiation Research; (USA)

DOI:https://doi.org/10.2307/3577784· OSTI ID:6920024

Lesiak, K B; Wheeler, K T ^[1]

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1. Wake Forest Univ. Medical Center, Winston-Salem, NC (USA)

When poly(dA), poly(dA-dT), and salmon testis DNA were gamma-irradiated under nitrogen, the major deoxyadenosine damage product (excluding liberated adenine) was identified as the alpha-anomer of deoxyadenosine. The yields of alpha-deoxyadenosine from poly(dA), poly(dA-dT), and salmon testis DNA irradiated with a dose of 500 Gy under anoxic conditions were 1.5, 1.3, and 1.3%, respectively. No alpha-deoxyadenosine was detected after irradiation under oxic conditions. The presence of nucleotides with the alpha-configuration at the anomeric carbon atom in the DNA chain may have a significant effect on its tertiary structure and possibly modify its biological activity.

• OSTI ID: 6920024
• Journal Information: Radiation Research; (USA), Journal Name: Radiation Research; (USA) Vol. 121:3; ISSN RAREA; ISSN 0033-7587
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADENOSINE
ALDEHYDES
ANADROMOUS FISHES
ANIMALS
ANOXIA
AQUATIC ORGANISMS
BIOSYNTHESIS
CARBOHYDRATES
CHEMICAL RADIATION EFFECTS
CHEMISTRY
DEOXYRIBOSE
DNA
ELECTROMAGNETIC RADIATION
FISHES
GAMMA RADIATION
IONIZING RADIATIONS
MAGNETIC RESONANCE
MOLECULAR STRUCTURE
MONOSACCHARIDES
NUCLEAR MAGNETIC RESONANCE
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
PENTOSES
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
RESONANCE
RIBOSIDES
SACCHARIDES
SALMON
SYNTHESIS
VERTEBRATES