Interaction of {alpha}-deoxyadenosine in template DNA with DNA polymerase: A novel mutational spectrum induced by {alpha}-deoxyadenosine
Conference
·
OSTI ID:134877
- Kyoto Institute of Technology (Japan); and others
DNA lesions produced by free radicals are suggested to play an important role in mutagenesis, carcinogenesis and aging. The molecular mechanism leading to such biological end points have been extensively studied by a number of researchers. In this study, we have focused our attention on {alpha}-deoxyadenosine ({alpha}-dA), which is a major adenine lesion in DNA {gamma}-irradiated under anoxic conditions. The anomerization of the N-glycosidic bond occurs via abstraction of the H1{prime} atom by hydroxyl radicals. Alpha-dA has been site-specifically incorporated into an oligodeoxyribonucleotide template to examine its interaction with DNA polymerase in vitro.
- Research Organization:
- New York Academy of Sciences, New York, NY (United States)
- OSTI ID:
- 134877
- Report Number(s):
- CONF-9307221--
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
55 BIOLOGY AND MEDICINE
BASIC STUDIES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
ADENOSINE
AMINO ACIDS
ANIMAL CELLS
BASES
BIOSYNTHESIS
CARCINOGENESIS
CHROMATOGRAPHY
DNA
DNA POLYMERASES
DNA SEQUENCING
GAMMA RADIATION
GENETIC RADIATION EFFECTS
LIFE CYCLE
MUTAGENESIS
MUTATIONS
NUCLEOSIDES
NUCLEOTIDES
STRUCTURE-ACTIVITY RELATIONSHIPS
BASIC STUDIES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
ADENOSINE
AMINO ACIDS
ANIMAL CELLS
BASES
BIOSYNTHESIS
CARCINOGENESIS
CHROMATOGRAPHY
DNA
DNA POLYMERASES
DNA SEQUENCING
GAMMA RADIATION
GENETIC RADIATION EFFECTS
LIFE CYCLE
MUTAGENESIS
MUTATIONS
NUCLEOSIDES
NUCLEOTIDES
STRUCTURE-ACTIVITY RELATIONSHIPS