skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Total lymphoid irradiation and discordant cardiac xenografts

Abstract

Total lymphoid irradiation can prolong concordant cardiac xenografts. The effects of total lymphoid irradiation in a discordant xenograft model (guinea pig to rat) were studied with and without adjuvant pharmacologic immunosuppression. Inbred Lewis rats were randomly allocated to one of four groups. Group 1 (n = 6) served as a control group and rats received no immunosuppression. Group 2 (n = 5) received triple-drug therapy that consisted of intraperitoneal azathioprine (2 mg/kg), cyclosporine (20 mg/kg), and methylprednisolone (1 mg/kg) for 1 week before transplantation. Group 3 animals (n = 5) received 15 Gy of total lymphoid irradiation in 12 divided doses over a 3-week period. Group 4 (n = 6) received both triple-drug therapy and total lymphoid irradiation as described for groups 2 and 3. Complement-dependent cytotoxicity assay was performed to determine if a correlation between complement-dependent cytotoxicity and rejection-free interval existed. Rejection was defined as cessation of graft pulsation and was confirmed by histologic test results. Only groups 1 and 2 showed a difference in survival (group 1, 6.9 +/- 1.0 minutes; group 2, 14.2 +/- 2.7 minutes, p = 0.02). Although total lymphoid irradiation did decrease complement-dependent cytotoxicity, linear regression revealed no correlation between complement-dependent cytotoxicity and graftmore » survival (coefficient of correlation, 0.30). Unlike concordant cardiac xenografts, total lymphoid irradiation with or without triple-drug therapy does not prolong graft survival.« less

Authors:
; ; ; ; ; ; ;  [1]
  1. (Tufts Univ. School of Medicine, Boston, MA (USA))
Publication Date:
OSTI Identifier:
6919638
Alternate Identifier(s):
OSTI ID: 6919638
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Heart Transplantation; (USA); Journal Volume: 9:1
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; HEART; TRANSPLANTS; IMMUNITY; RADIOINDUCTION; LYMPHATIC SYSTEM; BIOLOGICAL RADIATION EFFECTS; GRAFT-HOST REACTION; GUINEA PIGS; IMMUNOSUPPRESSIVE DRUGS; IRRADIATION; RATS; SURVIVAL TIME; ANIMALS; BIOLOGICAL EFFECTS; BODY; CARDIOVASCULAR SYSTEM; DRUGS; MAMMALS; ORGANS; RADIATION EFFECTS; RODENTS; VERTEBRATES 560152* -- Radiation Effects on Animals-- Animals

Citation Formats

Kaplan, E., Dresdale, A.R., Diehl, J.T., Katzen, N.A., Aronovitz, M.J., Konstam, M.A., Payne, D.D., and Cleveland, R.J. Total lymphoid irradiation and discordant cardiac xenografts. United States: N. p., 1990. Web.
Kaplan, E., Dresdale, A.R., Diehl, J.T., Katzen, N.A., Aronovitz, M.J., Konstam, M.A., Payne, D.D., & Cleveland, R.J. Total lymphoid irradiation and discordant cardiac xenografts. United States.
Kaplan, E., Dresdale, A.R., Diehl, J.T., Katzen, N.A., Aronovitz, M.J., Konstam, M.A., Payne, D.D., and Cleveland, R.J. Mon . "Total lymphoid irradiation and discordant cardiac xenografts". United States. doi:.
@article{osti_6919638,
title = {Total lymphoid irradiation and discordant cardiac xenografts},
author = {Kaplan, E. and Dresdale, A.R. and Diehl, J.T. and Katzen, N.A. and Aronovitz, M.J. and Konstam, M.A. and Payne, D.D. and Cleveland, R.J.},
abstractNote = {Total lymphoid irradiation can prolong concordant cardiac xenografts. The effects of total lymphoid irradiation in a discordant xenograft model (guinea pig to rat) were studied with and without adjuvant pharmacologic immunosuppression. Inbred Lewis rats were randomly allocated to one of four groups. Group 1 (n = 6) served as a control group and rats received no immunosuppression. Group 2 (n = 5) received triple-drug therapy that consisted of intraperitoneal azathioprine (2 mg/kg), cyclosporine (20 mg/kg), and methylprednisolone (1 mg/kg) for 1 week before transplantation. Group 3 animals (n = 5) received 15 Gy of total lymphoid irradiation in 12 divided doses over a 3-week period. Group 4 (n = 6) received both triple-drug therapy and total lymphoid irradiation as described for groups 2 and 3. Complement-dependent cytotoxicity assay was performed to determine if a correlation between complement-dependent cytotoxicity and rejection-free interval existed. Rejection was defined as cessation of graft pulsation and was confirmed by histologic test results. Only groups 1 and 2 showed a difference in survival (group 1, 6.9 +/- 1.0 minutes; group 2, 14.2 +/- 2.7 minutes, p = 0.02). Although total lymphoid irradiation did decrease complement-dependent cytotoxicity, linear regression revealed no correlation between complement-dependent cytotoxicity and graft survival (coefficient of correlation, 0.30). Unlike concordant cardiac xenografts, total lymphoid irradiation with or without triple-drug therapy does not prolong graft survival.},
doi = {},
journal = {Journal of Heart Transplantation; (USA)},
number = ,
volume = 9:1,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 1990},
month = {Mon Jan 01 00:00:00 EST 1990}
}