Xenograft survival in two species combinations using total-lymphoid irradiation and cyclosporine

Journal Article · Sun Feb 01 04:00:00 EST 1987 · Transplantation; (United States)

DOI:https://doi.org/10.1097/00007890-198702000-00002· OSTI ID:6645684

Knechtle, S J; Halperin, E C; Bollinger, R R

Total lymphoid irradiation (TLI) has profound immunosuppressive actions and has been applied successfully to allotransplantation but not xenotransplantation. Cyclosporine (CsA) has not generally permitted successful xenotransplantation of organs but has not been used in combination with TLI. TLI and CsA were given alone and in combination to rats that were recipients of hamster or rabbit cardiac xenografts. Combined TLI and CsA prolonged survival of hamster-to-rat cardiac xenografts from three days in untreated controls to greater than 100 days in most recipients. TLI alone significantly prolonged rabbit to rat xenograft survival with doubling of survival time. However, combined treatment did not significantly prolong rabbit-to-rat cardiac xenograft survival compared with TLI alone. The hamster and rat are phylogenetically closely related. Transplants from hamsters to rat are concordant xenografts since the time course of unmodified rejection is similar to first-set rejection of allografts. Although the rabbit-to-rat transplant is also between concordant species (average survival of untreated controls: 3.2 days) the rabbit and rat are more distantly related. These results suggest that TLI is an effective immunosuppressant when applied to cardiac xenotransplants in these animal models; that the choice of species critically affects xenograft survival when TLI and/or CsA are used for immunosuppression; and that the closely related species combination tested has markedly prolonged (greater than 100 days) survival using combined TLI and CsA.

• Research Organization: Duke Univ. Medical Center, Durham, NC
• OSTI ID: 6645684
• Journal Information: Transplantation; (United States), Journal Name: Transplantation; (United States) Vol. 2; ISSN TRPLA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CARDIOVASCULAR SYSTEM
HAMSTERS
HEART
IMMUNOSUPPRESSION
LYMPHATIC SYSTEM
MAMMALS
ORGANS
RABBITS
RADIATION EFFECTS
RATS
RODENTS
SURVIVAL TIME
SYNERGISM
TRANSPLANTS
VERTEBRATES