Prolonged heart xenograft survival using combined total lymphoid irradiation and cyclosporine

Journal Article · Thu May 01 04:00:00 EDT 1986 · J. of Heart Transplant.; (United States)

OSTI ID:6087506

Knechtle, S J; Halperin, E C; Saad, T; Bollinger, R R

Total lymphoid irradiation and cyclosporine have profound immunosuppressive properties and permit successful heart allotransplantation. Cyclosporine used alone has not permitted consistently successful transplantation between species in all cases. Total lymphoid irradiation has not been applied to xenotransplantation. The efficacy of total lymphoid irradiation alone and in combination with cyclosporine was examined using an animal model of heart xenotransplantation. Heterotopic heart transplants were performed using inbred Syrian hamsters as donors and Lewis rats as recipients. Total lymphoid irradiation was administered preoperatively over 3 weeks for a total dose of 15 gray. Cyclosporine was started on the day of surgery and was given as a daily intramuscular injection of 2.5, 5, or 10 mg/kg/day until rejection was complete. Neither total lymphoid irradiation nor cyclosporine alone markedly prolonged graft survival. However, combined total lymphoid irradiation and cyclosporine, 5 or 10 mg/kg/day, dramatically prolonged graft survival to greater than 100 days in most recipients. There were no treatment-related deaths. In conclusion, combined total lymphoid irradiation and cyclosporine permit successful long-term survival of heart xenotransplants in this hamster-to-rat model.

• Research Organization: Duke Univ. Medical Center, Durham, NC
• OSTI ID: 6087506
• Journal Information: J. of Heart Transplant.; (United States), Journal Name: J. of Heart Transplant.; (United States) Vol. 5:3; ISSN JHTRE
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL MODELS
BODY
CARDIOVASCULAR SYSTEM
DRUGS
GRAFT-HOST REACTION
HAMSTERS
HEART
IMMUNOSUPPRESSION
IMMUNOSUPPRESSIVE DRUGS
IRRADIATION
LYMPHATIC SYSTEM
MAMMALS
MUSCLES
MYOCARDIUM
ORGANS
RADIOSENSITIVITY EFFECTS
RATS
RODENTS
SURVIVAL TIME
SYNERGISM
TRANSPLANTS
VERTEBRATES