Ligand exchange chromatography of free amino acids and proteins on porous microparticulate zirconium oxide

Journal Article · Wed Jan 01 04:00:00 EST 1992 · Journal of Liquid Chromatography; (United States)

DOI:https://doi.org/10.1080/10826079208018302· OSTI ID:6915653

Blackwell, J A ^[1]; Carr, P W ^[2]

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1. 13M Co., Specialty Adhesives and Chemicals Div., St. Paul, MN (United States)
2. Dept. of Chemistry, Inst. for Advanced Studies in Bioprocess Technology, Univ. of Minnesota, Minneapolis, MN (United States)

The Lewis acid sites present on the underlying zirconium oxide particles are responsible for the unusual elution sequence for amino acids on copper loaded, phosphated zirconium oxide supports reported in an earlier study. To more thoroughly examine the effect of these strong Lewis acid sites in this paper. The authors have studied ligand exchange chromatography on copper loaded zirconium oxide particles. It is shown here that carboxylate functional groups on amino acid solutes strongly interact with surface Lewis acid sites. Addition of competing hard Lewis bases to the eluent attenuates these specific interactions. The result is a chromatographic system with high selectivity which is also suitable for ligand exchange chromatography of proteins.

• OSTI ID: 6915653
• Journal Information: Journal of Liquid Chromatography; (United States), Journal Name: Journal of Liquid Chromatography; (United States) Vol. 15:9; ISSN 0148-3919; ISSN JLCHD8
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102 -- Chemical & Spectral Procedures
400201* -- Chemical & Physicochemical Properties
ALLOYS
AMINO ACIDS
CARBOXYLIC ACIDS
CATALYST SUPPORTS
CATALYTIC EFFECTS
CHALCOGENIDES
CHEMICAL REACTION KINETICS
CHROMATOGRAPHY
COPPER ADDITIONS
COPPER ALLOYS
HYDROGEN COMPOUNDS
INORGANIC ACIDS
ION EXCHANGE CHROMATOGRAPHY
KINETICS
LEWIS ACIDS
LIGANDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PHOSPHORUS ADDITIONS
PROTEINS
REACTION KINETICS
SEPARATION PROCESSES
TRANSITION ELEMENT COMPOUNDS
ZIRCONIUM COMPOUNDS
ZIRCONIUM OXIDES