OH-induced free radicals in uridine studied by a method combining ESR, spin-trapping, and liquid chromatography
Journal Article
·
· Radiat. Res.; (United States)
Free radicals produced by the reactions of OH radicals with uridine were investigated by a method combining ESR, spin-trapping, and liquid chromatography. A N2O-saturated aqueous solution of uridine, containing 2-methyl-2-nitrosopropane as a spin-trap, was X-irradiated and the resulting spin-adducts were separated by gel permeation chromatography and reverse-phase HPLC. ESR and uv-absorbance spectra obtained from the separated spin-adducts show that 5-yl and 6-yl radicals are produced by OH addition to the 5,6 double bond of the base moiety. It is also shown that radicals due to H abstraction from the sugar moiety at the C-4' and C-5' positions are produced.
- Research Organization:
- Hokkaido Univ., Sapporo, Japan
- OSTI ID:
- 6914854
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 1; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
AZINES
CHEMICAL RADIATION EFFECTS
CHEMISTRY
CHROMATOGRAPHY
ELECTROMAGNETIC RADIATION
ELECTRON SPIN RESONANCE
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
HYDROXYL RADICALS
IONIZING RADIATIONS
MAGNETIC RESONANCE
NITROSO COMPOUNDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
RADICALS
RESONANCE
RIBOSIDES
SEPARATION PROCESSES
URACILS
URIDINE
X RADIATION
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
AZINES
CHEMICAL RADIATION EFFECTS
CHEMISTRY
CHROMATOGRAPHY
ELECTROMAGNETIC RADIATION
ELECTRON SPIN RESONANCE
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
HYDROXYL RADICALS
IONIZING RADIATIONS
MAGNETIC RESONANCE
NITROSO COMPOUNDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
RADICALS
RESONANCE
RIBOSIDES
SEPARATION PROCESSES
URACILS
URIDINE
X RADIATION