Studies of spin-trapped radicals in. gamma. -irradiated aqueous solutions of thymidine-5'-monophosphate and cytidine-5'-monophasphate by liquid chromatography and ESR spectroscopy
- Hiroshima Univ.
Aqueous solutions of thymidine-5'-monophosphate and cytidine-5'-monophosphate were ..gamma..-irradiated with 2-methyl-2-nitrosopropane as a spin-trapping reagent. Liquid chromatography was applied to separate the stable spin-trapped radicals in the irradiated solutions. The eluate was passed through flow cells of ESR and uv detectors and collected by a fraction collector. The amount of residual sugar in each fraction was determined by the phenol-H/sub 2/SO/sub 4/ method. From the combined results of the ESR, uv, and sugar determinations, the structures of most of the trapped radicals were identified. The short-lived radicals which were trapped by 2-methyl-2-nitrosopropane were radicals on N-1, C-5, and N-3 positions of the base in the thymidine-5'-monophosphate and C-5 and C-6 base radicals in the cytidine-5'-monophosphate.
- OSTI ID:
- 5340263
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 72:1; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AQUEOUS SOLUTIONS
AZINES
BIOCHEMICAL REACTION KINETICS
CHEMICAL RADIATION EFFECTS
CHEMISTRY
CHROMATOGRAPHY
CYTIDINE
DISPERSIONS
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
KINETICS
MEASURING INSTRUMENTS
MIXTURES
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS COMPOUNDS
PYRIMIDINES
RADIATION CHEMISTRY
RADIATION DETECTORS
RADIATION EFFECTS
RADIATIONS
RADICALS
REACTION KINETICS
RIBOSIDES
SEPARATION PROCESSES
SOLUTIONS
SPECTROSCOPY
THYMIDINE