Rapid determination of nicotine in urine by direct thermal desorption ion trap mass spectrometry
Conference
·
OSTI ID:6909962
The measurement of nicotine and cotinine in physiological fluids (urine, blood serum, and saliva) is widely used as a means of assessing human exposure to environmental tobacco smoke (ETS). Although numerous analytical methods exist for these measurements, they generally involve extensive sample preparation which increases cost and decreases sample throughput. We report the use of thermal desorption directly into an ion trap mass spectrometer (ITMS) for the rapid determination of nicotine and cotinine in urine. A 1{mu}L aliquot of urine is injected into a specially designed inlet and flash vaporized directly into an ITMS through an open-split capillary restrictor interface. Isobutane chemical ionization is used to generate (M+H){sup +} ions of the analytes and collision induced dissociation is used to generate characteristic fragment ions which are used to confirm their identity. Quantification is achieved by integrating the ion current for the characteristic ions and comparing with an external working curve. Detection limits are approximately 50 pg per analyte and the sample turnaround time is approximately 3 minutes without the need for extensive sample preparation. 12 refs., 5 figs.
- Research Organization:
- Oak Ridge National Lab., TN (USA)
- Sponsoring Organization:
- DOHHS
- DOE Contract Number:
- AC05-84OR21400
- OSTI ID:
- 6909962
- Report Number(s):
- CONF-900724-7; ON: DE90009423
- Country of Publication:
- United States
- Language:
- English
Similar Records
Pharmacokinetics of nicotine in rats after multiple-cigarette smoke exposure
Quantitation of Cotinine in Nonsmoker Saliva Using Chip Based Nanoelectrospray Tandem Mass Spectrometry
Increased hepatic nicotine elimination after phenobarbital induction in the conscious rat
Journal Article
·
Wed Jun 15 00:00:00 EDT 1983
· Toxicol. Appl. Pharmacol.; (United States)
·
OSTI ID:5021261
Quantitation of Cotinine in Nonsmoker Saliva Using Chip Based Nanoelectrospray Tandem Mass Spectrometry
Journal Article
·
Sat Dec 31 23:00:00 EST 2005
· Journal of Analytical Toxicology
·
OSTI ID:1003124
Increased hepatic nicotine elimination after phenobarbital induction in the conscious rat
Journal Article
·
Sat Sep 15 00:00:00 EDT 1990
· Toxicology and Applied Pharmacology; (USA)
·
OSTI ID:6170824
Related Subjects
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
AEROSOLS
ALKALOIDS
AMINES
AUTONOMIC NERVOUS SYSTEM AGENTS
AZINES
AZOLES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CHARGED PARTICLES
CHEMICAL ANALYSIS
COLLOIDS
DETECTION
DISPERSIONS
DISSOCIATION
DRUGS
ENVIRONMENTAL EXPOSURE
HETEROCYCLIC COMPOUNDS
HUMAN POPULATIONS
IONS
MASS SPECTROMETERS
MATERIALS
MEASURING INSTRUMENTS
NICOTINE
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASYMPATHOLYTICS
PARASYMPATHOMIMETICS
POPULATIONS
PYRIDINES
PYRROLES
PYRROLIDINES
QUALITATIVE CHEMICAL ANALYSIS
RESIDUES
SMOKES
SOLS
SPECTROMETERS
TOBACCO
URINE
WASTES
400102* -- Chemical & Spectral Procedures
AEROSOLS
ALKALOIDS
AMINES
AUTONOMIC NERVOUS SYSTEM AGENTS
AZINES
AZOLES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CHARGED PARTICLES
CHEMICAL ANALYSIS
COLLOIDS
DETECTION
DISPERSIONS
DISSOCIATION
DRUGS
ENVIRONMENTAL EXPOSURE
HETEROCYCLIC COMPOUNDS
HUMAN POPULATIONS
IONS
MASS SPECTROMETERS
MATERIALS
MEASURING INSTRUMENTS
NICOTINE
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASYMPATHOLYTICS
PARASYMPATHOMIMETICS
POPULATIONS
PYRIDINES
PYRROLES
PYRROLIDINES
QUALITATIVE CHEMICAL ANALYSIS
RESIDUES
SMOKES
SOLS
SPECTROMETERS
TOBACCO
URINE
WASTES