Rapid determination of nicotine in urine by direct thermal desorption ion trap mass spectrometry

Conference · Mon Jan 01 00:00:00 EST 1990

OSTI ID:6909962

Wise, M B; Ilgner, R H; Guerin, M R

The measurement of nicotine and cotinine in physiological fluids (urine, blood serum, and saliva) is widely used as a means of assessing human exposure to environmental tobacco smoke (ETS). Although numerous analytical methods exist for these measurements, they generally involve extensive sample preparation which increases cost and decreases sample throughput. We report the use of thermal desorption directly into an ion trap mass spectrometer (ITMS) for the rapid determination of nicotine and cotinine in urine. A 1{mu}L aliquot of urine is injected into a specially designed inlet and flash vaporized directly into an ITMS through an open-split capillary restrictor interface. Isobutane chemical ionization is used to generate (M+H){sup +} ions of the analytes and collision induced dissociation is used to generate characteristic fragment ions which are used to confirm their identity. Quantification is achieved by integrating the ion current for the characteristic ions and comparing with an external working curve. Detection limits are approximately 50 pg per analyte and the sample turnaround time is approximately 3 minutes without the need for extensive sample preparation. 12 refs., 5 figs.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: DOHHS
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 6909962
• Report Number(s): CONF-900724-7; ON: DE90009423
• Resource Relation: Conference: 5. international conference on indoor air quality and climate, Toronto (Canada), 29 Jul - 3 Aug 1990
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
NICOTINE
DETECTION
URINE
QUALITATIVE CHEMICAL ANALYSIS
DISSOCIATION
ENVIRONMENTAL EXPOSURE
HUMAN POPULATIONS
IONS
MASS SPECTROMETERS
SMOKES
TOBACCO
AEROSOLS
ALKALOIDS
AMINES
AUTONOMIC NERVOUS SYSTEM AGENTS
AZINES
AZOLES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CHARGED PARTICLES
CHEMICAL ANALYSIS
COLLOIDS
DISPERSIONS
DRUGS
HETEROCYCLIC COMPOUNDS
MATERIALS
MEASURING INSTRUMENTS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASYMPATHOLYTICS
PARASYMPATHOMIMETICS
POPULATIONS
PYRIDINES
PYRROLES
PYRROLIDINES
RESIDUES
SOLS
SPECTROMETERS
WASTES
400102* - Chemical & Spectral Procedures