Quantitation of Cotinine in Nonsmoker Saliva Using Chip Based Nanoelectrospray Tandem Mass Spectrometry

Journal Article · Sun Jan 01 04:00:00 EST 2006 · Journal of Analytical Toxicology

DOI:https://doi.org/10.1093/jat/30.3.178· OSTI ID:1003124

Tomkins, Bruce A ^[1]; Van Berkel, Gary J ^[1]; Jenkins, Roger A ^[1]; Counts, Richard Wayne ^[1]

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1. ORNL

A new analytical procedure was developed for the quantitation of nonsmoker salivary cotinine. Small volumes of saliva were diluted with water, fortified with cotinine-d{sub 3} (internal standard), then passed through small extraction columns. The analyte and internal standard were eluted with 0.1% (v/v) acetic acid/acetonitrile. Aliquots of each extract were analyzed directly, without chromatographic separation, using chip-based (NanoMate{sup TM}) nanospray tandem mass spectrometry. The calculated detection limit was 0.49 ng cotinine/mL saliva. This method was used to quantify salivary cotinine collected from nonsmoking human subjects living in one of three environmental tobacco smoke (ETS) exposure categories or 'cells': 1. smoking home/smoking workplace; 2. smoking home/nonsmoking workplace; and 3. nonsmoking home/smoking workplace. Samples were collected during five sequential days, including Saturday, as part of a larger study to evaluate potential variability in exposure to ETS. Salivary cotinine measurements were made for the purpose of excluding misclassified smokers and for comparison with known levels of exposure to airborne nicotine in each exposure category. The concentrations observed were consistent with those reported from other large studies reported elsewhere. A non-parametric statistical test was applied to the data within each cell. No statistically significant differences were found between the mean cotinine concentrations collected on a weekday as compared to those collected on a weekend day. When the non-parametric test was applied to the three cells, a statistically significant difference was observed between cell 1 compared to cells 2 and 3. The salivary cotinine concentrations were thus statistically invariant over a five-day exposure period, and they were greatest under the conditions of smoking home and smoking workplace.

• Research Organization: Oak Ridge National Laboratory (ORNL)
• Sponsoring Organization: ORNL work for others
• DOE Contract Number: AC05-00OR22725
• OSTI ID: 1003124
• Journal Information: Journal of Analytical Toxicology, Journal Name: Journal of Analytical Toxicology Journal Issue: 3 Vol. 30; ISSN 0146-4760
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
99 GENERAL AND MISCELLANEOUS
BIOLOGICAL INDICATORS
Cotinine
EXTRACTION COLUMNS
HUMAN POPULATIONS
MASS SPECTROSCOPY
NICOTINE
QUANTITATIVE CHEMICAL ANALYSIS
SALIVA
SENSITIVITY
Saliva
TOBACCO SMOKES