Energetic effect of Vitreoscilla hemoglobin expression in Escherichia coli. An on-line [sup 31]P NMR and saturation transfer study

Journal Article · · Biotechnology Progress; (United States)

OSTI ID:6909204

Chen, R; Bailey, J E ^[1]

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1. California Inst. of Technology, Pasadena, CA (United States)

A newly developed on-line [sup 31]P NMR system has been used to investigate the effect of Vitreoscilla hemoglobin (VHb) expression on the steady-state level of ATP in Escherichia coli under oxygen-limited conditions. The VHb-expressing strain GRO21 exhibited a higher growth rate on a minimal fed-batch medium after the onset of oxygen limitation and maintained the same level of ATP pool and transmembrane [Delta]pH as the slower growing wild-type strain MG1688, indicating that the net ATP accumulation rate (the rate at which ATP increases per unit volume of culture per unit time) was enhanced in the VHb-expressing strain. Further direct evidence of the enhanced flux of P[sub 1]-ATP (catalyzed by ATPase) in GRO21 was obtained in a saturation transfer study on respiring cells. VHb-expressing cells respiring on succinate exhibited a P[sub 1]-ATP flux 30 [+-] 5% higher than that of an otherwise isogenic strain that did not express VHb. 17 refs., 3 figs.

• OSTI ID: 6909204
• Journal Information: Biotechnology Progress; (United States), Vol. 10:4; ISSN 8756-7938
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE
ESCHERICHIA COLI
GENETIC ENGINEERING
GROWTH
RESPIRATION
ATP
CATALYSIS
CELL CULTURES
ENZYMES
HEMOGLOBIN
MATHEMATICAL MODELS
NUCLEAR MAGNETIC RESONANCE
OXYGEN
PH VALUE
BACTERIA
BIOTECHNOLOGY
CARBOXYLIC ACIDS
ELEMENTS
GLOBINS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
MAGNETIC RESONANCE
MICROORGANISMS
NONMETALS
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
RESONANCE
400102* - Chemical & Spectral Procedures
550400 - Genetics
550200 - Biochemistry
550300 - Cytology
550700 - Microbiology
990200 - Mathematics & Computers